FastQCFastQC Report
Tue 4 Jul 2023
SRR5047770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5047770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147874090
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA75773305.124176926464941Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG26297711.778385246529666No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA19225441.3001222864668178No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA16419981.1104027757668702No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC14484010.979482612538816No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA8458780.5720258363043856No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC7970690.5390187016535487No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC6804620.46016310227166907No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA6601510.4464277683805189No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT6315420.42708090376076024No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCT4869760.3293180029036865No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA4450390.30095806506738265No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC4428350.29946760788181354No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG4263920.2883480128263173No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG3580760.2421492500816066No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC3401030.22999499100890497No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3400630.22996794096923945No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT3238010.21897074734322963No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2944080.19909370194602719No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2920260.19748287208394655No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT2864350.19370195278970104No Hit
TTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAGA2787220.1884860288912006No Hit
TGAGCGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC2772010.1874574511329199No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCA2736810.18507704764235575No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT2708240.18314499855924726No Hit
TTGCATTTATCAGTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAAT2582060.1746120635467647No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG2531810.171213902313786No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT2479550.16767981463148818No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2401290.16238747437093273No Hit
TGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCCTGTAGGCACCATCAA2351430.1590156869266279No Hit
ATTTATCAGTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAGAT2331220.15764898367252841No Hit
AAAACCTGCATTAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATCG2221620.1502372728041809No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA2211650.14956305056551827No Hit
TGAGCGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCA2178780.14734021355600566No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2134350.14433563040016No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA2059670.13928538799461082No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG2058320.13919409411073974No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT1999890.13524275956660156No Hit
TGAATACAAGCTTGGGCTGCAGGTCTGTAGGCACCATCAATAGATCGGAA1883250.1273549680001412No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1844000.12470068285796382No Hit
TGAATACAAGCTTGGGCTGCAGGCTGTAGGCACCATCAATAGATCGGAAG1833500.1239906193167444No Hit
TGAATACAAGCTTGGGCTGCAGGTCCTGTAGGCACCATCAATAGATCGGA1830320.12377557150140366No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGAT1796010.12145535434909523No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1775090.1200406372745895No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC1775010.12003522726665639No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1753210.11856100010488653No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1675510.11330652989986278No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1630490.11026204793551055No Hit
AAACCTGCATTAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATCGG1623680.10980152101020538No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1509860.1021044322233868No Hit
TGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1503390.10166689783179732No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGAT1495790.10115294707815277No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1491740.10087906542653956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)