FastQCFastQC Report
Mon 10 Jul 2023
SRR5047771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5047771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215341454
Total Bases10.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA83958543.898856371611571Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC25212901.170833554416327No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA23392791.0863115097198146No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA21616031.0038025470005418No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG21195470.9842726333592974No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC21178730.98349526329473No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC18100450.8405464746235065No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG16551610.768621632878916No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG16492520.7658776187143234No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT12937910.6008090759896142No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA12767290.5928858453793109No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA12637190.586844277553731No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG11794050.5476906457592694No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA10616510.49300818782434713No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT10471370.4862681943254642No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG10060150.4671720104574013No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT9760680.45326525936803597No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG9530700.4425854763662922No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA8855890.411248732443313No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT8519540.3956293524422845No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA7908310.3672451287525903No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA7844640.364288429110356No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT7797200.3620854162153098No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC7760320.36037278730364664No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC7386050.342992482998652No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC7114710.33039202939532486No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG6979290.3241034120629649No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGAT5303140.2462665641702224No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA5068300.23536109308521713No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA4972530.23091373758440398No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC4932030.22903300355722497No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4496590.20881209430303188No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA4212360.19561305646241248No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG4173730.19381916126562423No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT4164000.1933673207203291No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4081770.18954873407699754No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA3837620.17821092635512714No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC3621390.1681696641650799No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAAT3597910.16707930280808822No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3504790.16275500768189297No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATC3473040.16128060508033906No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGCTGTAGGCACCATCAATAG3457420.16055524543825175No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3371640.15657180433080944No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3277800.1522140739330199No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC2942420.13663973867288925No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG2929570.13604301195068552No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC2878200.13365749819818715No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2858430.13273942136566053No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCA2851220.13240460427094544No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC2722390.12642201254942767No Hit
TGAGCGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC2682840.12458539450560224No Hit
TGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC2458190.11415312538941061No Hit
TTGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCA2452510.11388935824683341No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC2447740.11366784957252123No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2358430.10952048275851244No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT2300390.10682522836499468No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA2266860.10526816634199934No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2254900.10471276933051636No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2184520.10144447153217419No Hit
TGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT2157560.10019250636247679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)