FastQCFastQC Report
Mon 3 Jul 2023
SRR5047772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5047772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125334312
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA71743285.724153175229461Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC24425381.9488182932699227No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC22123131.7651295680308199No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA18690251.4912317067651832No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC17752681.4164261738637063No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA12976621.035360532397545No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG11374290.9075160519491263No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG10374890.8277773128877909No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA9566160.7632514869511551No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG8192410.6536446300515057No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG7800460.6223722678590999No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA7089540.5656503703471081No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT7057680.5631083689197576No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT6861880.5474861504804845No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT6848810.5464433394743492No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA6738550.5376460677424072No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG6406970.5111904232577588No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA5807330.4633471798209576No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG5190320.41411804295060073No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG5177940.41313028470607477No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG4984810.3977210965182464No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4958870.3956514318281813No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG4526570.36115967988079756No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA4508050.3596820318445599No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT4434880.3538440455156446No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT4112260.32810328906580666No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA4086660.3260607518234911No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGCTGTAGGCACCATCAATAG4073230.3249892176373857No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC4019410.3206951022318613No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3975340.3171789062838595No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC3919710.31274037711237446No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3894210.3107058185311617No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC3282000.26185965739373906No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGAT3265300.26052722098957226No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC3008450.24003402994704276No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCAT2981990.23792287621924313No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2966790.2367101197316183No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2580700.20590530707983618No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT2246280.17922306861986845No Hit
TTGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCA2143670.17103616446229028No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2039490.1627239953253982No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1937150.15455863355279756No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAAT1919930.15318470811089624No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATC1863120.14865203073839828No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1833890.14631986809805125No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1790030.14282042733836525No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1725710.13768855251704737No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1715380.13686435682512862No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1634800.13043515170849623No Hit
TGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT1582700.12627826927393992No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC1573260.12552508366583606No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1569920.1252585963850027No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC1522890.1215062320683581No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1510780.12054001620880961No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1501890.1198307132367711No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1484690.11845838352709033No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1457720.11630653862766645No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGAT1446390.11540255632471977No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA1440770.11495415557074268No Hit
TTGTAAGCCGGAGATGAACTGTAGGCACCATCAATAGATCGGAAGAGCAC1431930.11424884192925557No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1393630.111193014726885No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC1357840.10833745191819459No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC1357530.10831271806877593No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1349040.10763532974114862No Hit
TTGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCAT1341750.10705368534675486No Hit
GAGACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCA1320370.10534784760297723No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCA1277520.10192899132042949No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1255290.1001553349572781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)