FastQCFastQC Report
Mon 3 Jul 2023
SRR5047773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5047773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126120747
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1788317314.179406184455917Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA11686100.9265803032390857No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC11332930.8985777732508989No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC11093170.879567419625258No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA9935280.7877593684090691No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC9160440.7263230053656438No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG8549270.6778638886431588No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG8031290.6367937227647407No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT7268570.5763183435632522No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG6432140.509998565105232No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT6246080.49524603592777644No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA6071220.48138154462405774No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG5979910.4741416572802253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG5895790.4674718585356936No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC5645100.4475948750922003No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT5627120.446169257148469No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA5487670.4351123927294849No Hit
ACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC5465650.4333664468384413No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5242120.4156429552387602No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC5176670.4104534839141097No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA5127230.4065334310143279No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4907580.38911758110662No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4775060.3786101901220106No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC4141290.3283591398328778No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA3967060.31454460065955686No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT3940910.31247119080257274No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3766840.2986693378845909No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3759850.29811510710446393No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT3621180.2871200881802579No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3607760.28605602851369094No Hit
ACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3443650.2730438949905681No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3142580.24917232689717575No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCAT2957450.23449353657887867No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2957430.23449195079696125Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC2675530.21214035467138487No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCA2526260.20030487133096347No Hit
GGGAGAGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2508220.19887449604148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2455400.1946864459976597No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2396900.19004803388930133No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2282680.1809916333591015Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA2280090.18078627460079982No Hit
TGAGCGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC2235610.1772594956165301No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2129970.16888339552888948No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2110140.1673110927577998No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2106050.16698680035569405No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC2056430.16305247541865572No Hit
TGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1978720.15689091977864675No Hit
AAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1950820.1546787540038912Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1888380.14972794285780752No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGAT1883450.14933704761517153No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1834300.14543998855319182No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1705250.13520773073124914Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1658010.13146211384237996No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1562000.12384956774796141Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1546120.12259045690555576No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1515910.12019513331934198No Hit
TTGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCA1500700.11898914617116882No Hit
ATGTTTAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1464930.11615297521192132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT1429730.11336199903731939No Hit
TCAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATAGA1419660.11256355784191477No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATC1381910.10957039447284593No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1363320.10809641018063426No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTATCT1339640.10621884439044751TruSeq Adapter, Index 2 (97% over 43bp)
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1338920.10616175624142156Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT1315010.10426595395918485No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAGTCAC1264710.10027771243695535Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)