FastQCFastQC Report
Tue 4 Jul 2023
SRR5047774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5047774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155643960
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA129755728.336701276426018Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC25979381.669154395711854No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC23685341.521764159688561No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA20793231.3359484042940053No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC20675071.3283567187573486No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA13570520.8718950610097559No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG12526100.8047919109742517No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG11604100.7455541480697355No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG10073060.6471860520639541No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA9714140.624125729003554No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG9451490.6072506764798326No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT8755540.5625364453590104No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT8518510.5473074573533082No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG8391240.5391304615996663No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG8187100.5260146297999614No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCAT7948640.5106937654374767No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA7904320.5078462408692249No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA7641550.4909634784414378No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA6836090.439213317368692No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT6635240.4263088654387874No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC6483220.41654170197160234No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA6391960.41067831992966514No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC6201090.3984150750212215No Hit
TGAGCGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC6118510.39310937604003393No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA5854470.3761450171275519No Hit
TGGACGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC5789730.3719855238841263No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG5776850.37115799418107837No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT5740670.3688334581052808No Hit
ACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4292500.2757896933488457No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC4257200.27352169656952957No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC4113350.2642794490708152No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3601930.2314211229269674Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG3528500.2267033041307867No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC3515850.22589055174386466No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3514320.22579225046702742No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA3477940.22345486455112037No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGCTGTAGGCACCATCAATAG3463580.22253224603126262No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3070960.19730672491242193No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGAT2985930.19184361538989372No Hit
TTGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATC2974220.191091257251486No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2896840.18611965411314388No Hit
ACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2628220.16886103386215565No Hit
TTGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCA2617550.16817549489231703No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA2605400.16739486710566862No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA2564530.16476900227930463No Hit
TGGACGCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCA2435130.15645515572849727No Hit
GGGAGAGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2433600.15635685445166006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA2309860.14840665837594982No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2279830.14647725488351748No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG2262010.14533233412976643No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG2254150.14482733541346546No Hit
TGAGCGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC2240790.14396896609415488No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATC2135930.13723179492477575No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGAT2127650.13669981154424496No Hit
TTGACGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCAT2046780.1315039786959931No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2018620.12969472120858402No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGCTATCT1985450.12756357522643347TruSeq Adapter, Index 5 (97% over 37bp)
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1973840.1268176420080805No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1947460.12512274809764543Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1914970.12303529157186696No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCT1752030.1125665268347066No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1725600.11086842046424417No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC1694140.10884714061502933No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1682470.10809735244464354No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1663280.1068644102861428Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC1640220.10538282372152444No Hit
ATCCCATCCTCGTCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1625680.10444864034556817No Hit
TGACGGCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC1615420.10378944354795393No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCAT1603810.10304351032960096No Hit
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATAGATCGG1576890.10131392185087042No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAAT1568920.10080185572250926No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)