FastQCFastQC Report
Mon 3 Jul 2023
SRR5090708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5090708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191796596
Total Bases14.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGGA131975306.881003247836579No Hit
AACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG79785684.159911159215777No Hit
TACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG68156543.5535844442202715No Hit
CCTGCGGTTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA47744012.4893043461522124No Hit
ACACTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGA21463971.1191006747585865No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC15319180.7987201190995068No Hit
ACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGAA12173630.6347156442755636No Hit
CACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG10278510.5359067999309017No Hit
AACACTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG10000740.5214242696987177No Hit
GGTTAGAGCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC8794080.4585107443721264No Hit
TACACTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG8212280.42817652509328163No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACCTGTAGGCACCATCAATAGATCG8051260.41978117275866567No Hit
AACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGA7267220.37890244934274014No Hit
TACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGA6308560.328919289057664No Hit
ACACTGAGGCGTGACGACGAGGCACTCTGTAGGCACCATCAATAGATCGG5496530.28658120710338364No Hit
TAACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCG5442980.2837891867486533No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGTAGGCACCATCAATAGA5436010.2834257809247042No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTGTAGGCACCATCAATAGATCG5290670.27584796134755174No Hit
ACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAG4830510.2518558775672953No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCTGTAGGCACCATCAATAGA4574950.23853134494628883No Hit
ACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATTGGA4509430.23511522592403045No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA4234200.22076512765638448No Hit
GACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG4168490.21733910230607012No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACTGTAGGCACCATCAATAGAT4111480.21436668250358312No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA4109650.2142712689228332No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG3909620.2038419910226144No Hit
TCCTGCGGTTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC3845250.2004858313543792No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCCTGTAGGCACCATCAATAGA3730880.19452274325035465No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGCTGTAGGCACCATCAATAGATC3380460.17625234600096865No Hit
ACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATTAATAGATCGGA3318540.17302392582608714No Hit
CACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGGAA3281250.17107967859867546No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATAGA3159830.1647490135852046No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATAGATC2992070.15600224729744422No Hit
ACACTGAGGCGTGACGACGAGGCCTGTAGGCACCATCAATAGATCGGAAG2984380.1556013017040198No Hit
AACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATTGG2922710.15238591617131725No Hit
GCTGATATGGCTCAGTTGGTAGAGCGCACCTGTAGGCACCATCAATAGAT2877280.15001726099455906No Hit
TACACTGAGGCGTGACGACGAGGCACTCTGTAGGCACCATCAATAGATCG2876170.14995938718328453No Hit
ACGATGTCTACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATAGATCGG2872370.14976126062216452No Hit
AACACTGAGGCGTGACGACGAGGCCTGTAGGCACCATCAATAGATCGGAA2817330.14689155380004762No Hit
ACGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2778830.14488421890448983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCTGTAGGCACCATCAATAGA2733530.1425223417416647No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGAGCTGTAGGCACCATCAATAGATC2529790.1318996297515103No Hit
GCTGATATAGCTCAGTTGGTAGAGCGCACCTGTAGGCACCATCAATAGAT2515740.13116708286105347No Hit
CGGAGGTTCGAATCCTCCCGGATGCTGTAGGCACCATCAATAGATCGGAA2515320.13114518466219285No Hit
CCTGCGGTTGGATCACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2511640.13095331472931876No Hit
TACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATTGG2431250.12676189519025666No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACTGTAGGCACCATCAA2400070.12513621461769844No Hit
ACACTGAGGCGTGACGACGAGGCACCTGTAGGCATCATCAATAGATCGGA2380410.12411117035674606No Hit
ACACTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATAGATCGGAA2361550.12312783695076632No Hit
ACACTGAGGCGTGACGACGAGGCACCTGTAGGCACTATCAATAGATCGGA2251780.1174045862628344No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAG2239550.11676693156744034No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCTGTAGGCACCATCA2233350.11644367244140244No Hit
AACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATTAATAGATCGG2145290.11185235008029026No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC2140690.11161251266419765No Hit
AACTTGCCCCGAGATGAGTTCTCCCTGACTGTAGGCACCATCAATAGATC2135220.11132731469332229No Hit
ACACTGAGGCGTGACGACGAGGCACCTGTAGGTACCATCAATAGATCGGA2124200.11075274766607433No Hit
GCGCCCTTAGCTCAGTTGGATAGAGCAACGCTGTAGGCACCATCAATAGA2072870.10807647493389298No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2038210.10626935214220382No Hit
GGTGAGGTGTCCGAGTGGCTGAAGGAGCACCTGTAGGCACCATCAATAGA2035270.1061160647501794No Hit
TACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATTAATAGATCGG1971890.10281152226497284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)