FastQCFastQC Report
Mon 3 Jul 2023
SRR5090709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5090709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174834326
Total Bases13.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG73691524.214934314443492No Hit
AACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG54113283.0951176029357073No Hit
ACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGGA21985241.2574899050430177No Hit
AGCGCACGCAGGCGGTCTGTCAAGTCGGATGCTGTAGGCACCATCAATAG16112370.9215793241883177No Hit
TACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGA15252520.872398478545912No Hit
ATCGCGCGGATGCCCTGCCTGGGGTTGAAGCGCTGTAGGCACCATCAATA14573660.833569719026457No Hit
CACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG13411560.7671010783088442No Hit
TCGCGCGGATGCCCTGCCTGGGGTTGAAGCGCTGTAGGCACCATCAATAG12496770.7147778291546707No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC12425280.7106888151929616No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA12020950.6875623497413202No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG11805560.6752426866106374No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAA11106520.6352596915093206No Hit
AACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGA11090300.6343319560713724No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA10720540.6131827911184902No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA10631420.6080853939403181No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCA8759780.5010331895579818No Hit
CACAGGTTCGAATCCCGTCGTAGCCCTGTAGGCACCATCAATAGATCGGA8620130.4930456276646727No Hit
TACACTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG6972380.3987992609643486No Hit
TTCGCGCGGATGCCCTGCCTGGGGTTGAAGCGCTGTAGGCACCATCAATA6818660.39000693719607443No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATAGATC6786020.38814002691897015No Hit
AACACTGAGGCGTGACGACGAGGCCTGTAGGCACCATCAATAGATCGGAA6142250.3513183103414143No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA5914160.3382722452340395No Hit
GGAGCGGTAGTTCAGTTGGTTAGAATACCTGCCCTGTAGGCACCATCAAT5742280.32844122383610186No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC5172810.295869244807224No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT5019330.28709064832039904No Hit
GACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGG4881560.27921061679844267No Hit
AGCGCACGCAGGCGGTCTGTCAAGTCGGCTGTAGGCACCATCAATAGATC4691060.26831458714806383No Hit
ACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGTGCTGTAGGCACCATCAA4454460.25478177551929937No Hit
TACACTGAGGCGTGACGACGAGGCTGTAGGCACCATCAATAGATCGGAAG4441660.2540496538420035No Hit
TACACTGAGGCGTGACGACGAGGCCTGTAGGCACCATCAATAGATCGGAA4401750.25176692133099765No Hit
ACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGAA4316860.24691146748836956No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATAGAT4307040.24634979288906916No Hit
AACACTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGG4292520.24551929236138673No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCCTGTAGGCACCATCA4145540.2371124764138136No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATAGA4133360.23641581688026184No Hit
ACGATGTCTACTTGGAGGTTGTGCCCTGTAGGCACCATCAATAGATCGGA3575780.2045239102531845No Hit
TAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGCTGTAGGCACCATCAAT3358700.19210758418229612No Hit
AACACTGAGGCGTGACGACGAGGCTGTAGGCACCATCAATAGATCGGAAG3341160.1911043486963767No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCCTGTAGGCACCATCAATA3312610.18947137417397084No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA3312380.18945821886258193No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA3308590.18924144220969513No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3243510.18551906105669433No Hit
AAGCGCACGCAGGCGGTCTGTCAAGTCGGATGCTGTAGGCACCATCAATA3138720.1795253867938954No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAAT3020460.17276126885975468No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC3005100.17188272284699974No Hit
ACACTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGA2906740.16625682533302985No Hit
ACACATGCAAGTCGAACGGTAACAGGAAGCAGCCTGTAGGCACCATCAAT2880530.16475769180475464No Hit
CGCACGCAGGCGGTCTGTCAAGTCGGATGCTGTAGGCACCATCAATAGAT2793560.15978326818956592No Hit
TACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATTGG2777280.15885210093125535No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATAGATCGGA2692010.1539749122263325No Hit
ACGGCGCGAGCAGGTGCGAAAGCAGGTCATAGCTGTAGGCACCATCAATA2627310.1502742659356264No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTCTGTAGGCACCATCAA2597740.14858295046706105No Hit
CACACTGAGGCGTGACGACGAGGCACTGTAGGCACCATCAATAGATCGGA2549610.14583005856641676No Hit
AGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATCA2486200.14220319641350063No Hit
ACGCCGTAGCGCCGATGGTAGTGTGGGGCTGTAGGCACCATCAATAGATC2462930.14087222208297928No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT2415400.13815364838595826No Hit
ACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAG2395530.1370171438759686No Hit
AAGACTGACTAAGCATGTAGTACCGCTGTAGGCACCATCAATAGATCGGA2320890.1327479593452375No Hit
TAGTCGAACGGTAACAGGAAGCAGCCTGTAGGCACCATCAATAGATCGGA2137800.12227575950960567No Hit
AACACTGAGGCGTGACGACGAGGCACCTGTAGGCACCATCAATAGATTGG2109310.12064621680756216No Hit
TCTAGCCATGGGCAGGTTGAAGGTTGGGCTGTAGGCACCATCAATAGATC2080950.11902410971630366No Hit
GGTGGCTATAGCTCAGTTGGTAGAGCCCTGGACTGTAGGCACCATCAATA2024340.11578618720445091No Hit
CACAGGTTCGAATCCCGTCGTAGCCACCTGTAGGCACCATCAATAGATCG1995100.11411374674787833No Hit
ACGATGTCTACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATAGATCGG1978670.11317399993866194No Hit
CGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAATA1962650.11225770390192141No Hit
CGCGCGGATGCCCTGCCTGGGGTTGAAGCGCTGTAGGCACCATCAATAGA1954290.11177953693143759No Hit
ATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCCTGTAGGCACCATCA1940680.11100108567925042No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCCTGTAGGCACCATCAAT1916790.10963464920498507No Hit
CACAGGTTCGAATCCCGTCGTAGCCTGTAGGCACCATCAATAGATCGGAA1892770.10826077711993468No Hit
TTGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATAGA1848980.10575612022549852No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT1816160.10387891448730725No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGCTGTAGGCACCATCAATAGATC1805620.103276058043659No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCTGTAGGCACCATCA1788860.10231743622244982No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAG1785450.10212239443185772No Hit
TATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCCTGTAGGCACCATC1754400.10034642739435505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)