FastQCFastQC Report
Tue 4 Jul 2023
SRR5131986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5131986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63437281
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGATTCATGATAACTCGACGGATCGCAAAAAAAAAAAAAAAAAAA2641920.4164617332826733No Hit
CCGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAATGG2621710.4132759094766372No Hit
TCTGATGATTCATGATAACTCGACGGATCGCATAAAAAAAAAAAAAAAAA2497360.3936738713628032No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAA1778100.2802925932465485No Hit
AAGAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCGAAAAAAAAAAAAA1621770.2556493554633907No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCAAAAAAAAAAAAAAAAAA1373610.21653040268229656No Hit
CCGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAATGGA1364710.21512744217394814No Hit
CCGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAAGGA1250910.19718846398854958No Hit
CCGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAAGGG1159230.18273639439243936No Hit
GTAACCCATGCTATACTCCCAGGAAAAGCTCGAAAAAAAAAAAAAAAAAA1153420.18182052916170854No Hit
CCGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAAAGG1149680.1812309704761779No Hit
GTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAATGGAATTCTCGGG1142960.18017165647436875No Hit
CTCTGATGATTCATGATAACTCGACGGATCGCATAAAAAAAAAAAAAAAA1134770.1788806175346639No Hit
CGAGGGCACGTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAAATGGA1098850.17321833197737463No Hit
TGCTCTGATGATTCATGATAACTCGACGGATCGCATAAAAAAAAAAAAAA1068800.1684813698115466No Hit
TGCTCTGATGATTCATGATAACTCGACGGATCGCAAAAAAAAAAAAAAAA991060.15622674622514163No Hit
GCTCTGATGATTCATGATAACTCGACGGATCGCATAAAAAAAAAAAAAAA920980.14517961449198932No Hit
GCGGGTGCACCGCCGACCGACCTTGAAAAAAAAAAAAAAAAAAAATGGAA858220.13528637836795054No Hit
GCTCTGATGATTCATGATAACTCGACGGATCGCAAAAAAAAAAAAAAAAA808650.1274723612444865No Hit
TGGTCGGCTTGTCCCTTCGGTCGGAAAAAAAAAAAAAAAAAAAATGGAAT785120.12376318587803282No Hit
TTCTGATGATTCATGATAACTCGACGGATCGCAAAAAAAAAAAAAAAAAA765810.12071923448295334No Hit
GAAGGTCACGGCGAGACGAGCCGTTTAAAAAAAAAAAAAAAAAAAAGGAA755750.11913341620048312No Hit
GTCTGCCTGGGTGTCACAAAAAAAAAAAAAAAAAAATGGAATTCTCGGGG710930.11206817013484546No Hit
GCCAAGGATGTTTTCATTAATCAAGAACAAAAAAAAAAAAAAAAAAAAGG705910.11127683735373209No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCGATACGCAAAAAAAAAAAAAAAAAA670840.10574854240679073No Hit
TGGTCGGCTTGTCCCTTCGGTCGGAAAAAAAAAAAAAAAAAAAAAGGAAT656520.10349119471245938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)