FastQCFastQC Report
Thu 28 Sept 2023
SRR5172020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5172020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47403595
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCAAGGA29307686.182585941002998RNA PCR Primer, Index 1 (100% over 22bp)
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTG27578735.817856219554656No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCCCTGGAATTCTCGGGTG27547925.8113567125025005No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGG23447034.9462556584579715Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGCCTGGGAATACCGAGTGTCGTAGGCGTGGAATTCTCGGGTGCCAA23251634.9050351560889No Hit
AGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCCA15493353.2683913530186897No Hit
ACCTCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA14733743.1081482322174088No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCA10978072.315872878417766No Hit
GCGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCAAGG9541042.012724984254886Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA8255211.7414734051288725No Hit
GCGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAG6668911.4068363380456692No Hit
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCCCCCTGGAATTCTCGGGTG6432351.356932949916562No Hit
ACTGCCTGGGAATACCGAGTGTCGTAGGCGTTGGAATTCTCGGGTGCCA6368841.3435352318742912No Hit
CCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCTGGAATTCTCGGGTGC6290411.3269900732212399No Hit
CCTCCCGGGGCTACGCCTGCCTGAGCGTCGCTTGGAATTCTCGGGTGCC6205811.309143325522041No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGTGGAATTCTCGGGTGCCA3818170.8054600078327393No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGGAATTCTCGGGTGCCA3153610.6652681088849908No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCTGGAATTCTCGGGTGCCAA2781640.5867993767139391No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGC2766240.5835506779601842No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGC2712800.5722772713757258No Hit
CGGGGCTTCTCGGAGTCGGGTTGCTTGTGGAATTCTCGGGTGCCAAGGA2708910.571456658508706RNA PCR Primer, Index 1 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGCCTGAGCGTCGCTTGGAATTCTCGGGTGC2691180.5677164358525973No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGGAATTCTCGGGTGCCAA2359400.4977259636109877No Hit
CTCCCGGGGCTACGCCTGCCTGAGCGTCGCTTGGAATTCTCGGGTGCCA2340830.4938085392046742No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTG2260270.47681404754217477No Hit
GCCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGT2196240.4633066331783486No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCCGTGGAATTCTCGGGTGCCAAGG2119150.44704415350776666Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGC2001300.4221831698629608No Hit
CCTCCCGGGGCTACGCCTGCCTGAGCGTCGCCTGGAATTCTCGGGTGCC1924750.40603460560322485No Hit
CTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGGTGC1908610.40262980054571806No Hit
CCGGGGCTTCTCGGAGTCGGGTTGCTTGTGGAATTCTCGGGTGCCAAGG1867720.39400387249110536Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTTCTCGGAGTCGGGTTGCTTGGTGGAATTCTCGGGTGCCAAGG1863460.3931052064722096Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGGT1851710.3906264915139875No Hit
ACCTCCTGGGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAA1850390.3903480316208085No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGCTGGAATTCTCGGGTGCC1840510.38826380151125667No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTGGAATTCTCGGGTG1824050.3847914910251005No Hit
AGACTGCCTGGGAATACCGAGTGTCGTAGGCGTGGAATTCTCGGGTGCC1803970.38055552537734744No Hit
CGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCCAAGGA1782650.3760579761935777RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCC1697980.35819646168186187No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGTGGAATTCTCGGGTGC1638410.3456299042298374No Hit
TGACTCTAGATAACCTCGAGCCGATCGTGGAATTCTCGGGTGCCAAGGA1570430.3312892197311196RNA PCR Primer, Index 1 (100% over 22bp)
TTCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCTGGAATTCTCGGGTG1570160.3312322620256966No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGTGGAATTCTCGGGTGC1558770.3288294906747052No Hit
ATGACCCGCCGGGCAGCTCCCGGGAAACCCAAGTGGAATTCTCGGGTGC1386550.29249891279342843No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCTGGAATTCTCGGGTGCCAAGGAA1362040.2873284188678095RNA PCR Primer, Index 1 (100% over 23bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGGAATTCTCGGGTGCCAAG1340770.28284141740726626No Hit
CAGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGGTGGAATTCTCGGG1320390.2785421654201543No Hit
AGCGGCCGCCGGGTGTTATAGCCGCCGGGCCCGTGGAATTCTCGGGTGC1268390.26757253326461844No Hit
GTGACTCTAGATAACCTCGAGCCGATCGTGGAATTCTCGGGTGCCAAGG1265450.2669523271389016Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGCCTGGGAATACCGAGTGTCGTAGGCGCTGGAATTCTCGGGTGCCA1240520.26169323233818026No Hit
CCGGGGCTTCTCGGAGTCGGGTTGCTTGGTGGAATTCTCGGGTGCCAAG1218650.2570796581989193No Hit
CGCGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCAAG1167820.24635684276688297No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGC1148920.24236980338727473No Hit
AAATTACCCACTCCCGACCCGGGGAGGTAGTGTGGAATTCTCGGGTGCC1117390.23571840912065847No Hit
ACCTCCTGGGAATACCGGGTGCTGTAGGCTCTGGAATTCTCGGGTGCCA1089430.22982012229241264No Hit
GCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCCAAGG1082000.2282527306209582Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCCAA1072980.22634992135090176No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTG1025910.21642029470549648No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCGTGGAATTCTCGGGTGCCCAGGA1021440.21547732824904944RNA PCR Primer, Index 1 (95% over 22bp)
AGGCCGGTAGCGGCCCCCCGGCGCGCCGGACTGGAATTCTCGGGTGCCA989420.20872256629481373No Hit
ATCCCCGAATCCGGAGCGGCGGAGACGGGCGCCTGGAATTCTCGGGTGC983480.2074694967755083No Hit
CTTTGGTGACTCTAGATAACCTCGAGCCGATCGTGGAATTCTCGGGTGC980760.20689570063198792No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGGAATTCTCGGGTGCC928740.19592184938716145No Hit
GCGTCCGGTGAGCTCTCGCTGGCCCGTGGAATTCTCGGGTGCCAAGGAA922410.1945865076266895RNA PCR Primer, Index 1 (100% over 23bp)
AGGCCGGTAGCGGCCCCCCGGCGTGCCGGGCTGGAATTCTCGGGTGCCA886380.1869858182696903No Hit
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC882110.1860850427061492No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCCCGTGGAATTCTCGGGT863450.18214863239802803No Hit
TCCTCCCGGGGCTACGCCTGCCTGAGCGTCGCCTGGAATTCTCGGGTGC834720.17608791063209445No Hit
CGCGCCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAA833510.17583265573001372No Hit
TCGGGGGGCCCAAGTCCTTCTGATCGAGTGGAATTCTCGGGTGCCAAGG803930.16959262266923006Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCCCGGGGCTACGCCTGCCTGAGCGTCGCTTTGGAATTCTCGGGTGC790110.16667723196943185No Hit
ACCCGCCGGGCAGCTCCCGGGAAACCCAAGTGGAATTCTCGGGTGCCAA786420.16589880999531786No Hit
CGAGCGCCGTTCCGAAGGGACGGGCGATGTGGAATTCTCGGGTGCCAAG775420.16357831088549296No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCCTGGAATTCTCGGGTGCCAAGGA766240.16164174890111183RNA PCR Primer, Index 1 (100% over 22bp)
CGCCTCGGTGGGCTCGCGATAGCCGGCCGCCGCCCCTGGAATTCTCGGG765990.16158901028497943No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGGAATTCTCGGGTGC763380.16103841913255734No Hit
CTCCCGGGGCTACGCCTGCCTGAGCGTCGCCTGGAATTCTCGGGTGCCA746670.1575133700302688No Hit
GCGCCGGCTGAGGTGGGATCCCGGGGCTGGAATTCTCGGGTGCCAAGGA722630.15244202470297874RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGCCGCCGGGTGTTATAGCCGCCGGGCCCGGTGGAATTCTCGGGTG705620.14885368926133136No Hit
ACTGCCTGGGAATACCGAGTGTCGTAGGCCTGGAATTCTCGGGTGCCAA702890.1482777835731657No Hit
CCGGCTGAGGTGGGATCCCGGGGCGGTGGAATTCTCGGGTGCCAAGGAA658960.13901055394638318RNA PCR Primer, Index 1 (100% over 23bp)
ACCTCCTGGGAATACCGGGTGCTGTAGGCTATGGAATTCTCGGGTGCCA639330.13486951780766837No Hit
CCGGGGCTTCTCGGAGTCGGGTTGCTTGGGTGGAATTCTCGGGTGCCAA625470.131945688929289No Hit
AGGCCGGTCGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGTGCCA612900.12929399131015273No Hit
AAACTAACGAGGATTCCCTCAGTAACGTGGAATTCTCGGGTGCCAAGGA609580.1285936224879147RNA PCR Primer, Index 1 (100% over 22bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCCGTGTGGAATTCTCGGGTGCCA609330.12854088387178228No Hit
CGCCGGCTGAGGTGGGATCCCGGGGCGGCTGGAATTCTCGGGTGCCAAG601930.12697982083426373No Hit
GGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGCCA571310.12052039513036933No Hit
AAACTGCGAATGGCTCATTAAATCAGTTATGTGGAATTCTCGGGTGCCA561630.11847835591372341No Hit
CGGGGCTTCTCGGAGTCGGGTTGCTTGGGTGGAATTCTCGGGTGCCAAG542600.11446389245372635No Hit
TGTGAGGCCGGTAGCGGCCCCCCGGCGCGCCGGGCTGGAATTCTCGGGT518650.10941153302824395No Hit
ACCTCCTGGGAATACCGGGTGCTGTAGGCCTGGAATTCTCGGGTGCCAA513800.10838840387527571No Hit
CTTTGGTGACTCTAGATAACCTCGAGCCGTGGAATTCTCGGGTGCCAAG511490.10790109906221247No Hit
ACTGCCTGGGAATACCGAGTGTCGTAGGCTGGAATTCTCGGGTGCCAAG507160.10698766623079958No Hit
CCGGGGCTTCTCGGAGTCGGGTTGCTTGGGAATGTGGAATTCTCGGGTG496800.10480217797827358No Hit
ATGCCGACTCGCGATCCGGCGGCGTTATTGGAATTCTCGGGTGCCAAGG496750.1047916302550471Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA489420.1032453340300456No Hit
AAGAACGCAGCTAGCTGCGAGAATTAATGTGGAATTCTCGGGTGCCAAG479800.10121595208127147No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)