FastQCFastQC Report
Sun 2 Jul 2023
SRR5186136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5186136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61670104
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA8364211.3562827784431821Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT5755780.9333177060962958No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT4372220.7089691303260978No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT2873910.46601348361598355No Hit
TAGTCCGGTTCGACTCCGGAACGCGCCTCCACTGTAGGCACCATCAATAT2559060.4149595726318217No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC2043920.3314280125099189No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1836690.29782502069398165No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC1767900.2866705073174516No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1513290.245384700502532No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA1496190.24261188208795628No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC1462780.23719434622649574No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG1454810.23590198583093033No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1341520.217531658451557No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT1309160.21228438336993888No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA1195820.19390594833438257No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT1185610.19225036494182013No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA1174300.1904164131132323No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG1159300.18798411625834133No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1137490.18444755663132983No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG1089400.17664961291454934No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC1031500.16726094705467012No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT845710.13713451820999037No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA814480.13207047615810735No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATA773880.12548705933753573No Hit
AAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATAT769390.12475899181230504No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC762650.12366607975884068No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT757500.12283099117199477No Hit
TGTGCGGGTTCAAGTCCCGCTCCGGGTACCACTGTAGGCACCATCAATAT733850.11899606979744999No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATATCTC729580.11830367595942436No Hit
TTTCCCTGGTTCGAATCCAGGTACCCCAGCCACTGTAGGCACCATCAATA664030.10767453870355075No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG648760.10519846050527173No Hit
AGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTAT629190.10202512387525728No Hit
GCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATC628810.10196350568826673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)