FastQCFastQC Report
Tue 4 Jul 2023
SRR5186137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5186137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68702208
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT7875391.1463081361227867No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6278020.9138017805774161Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC4285910.6238387563904787No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC3919980.570575548314255No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA3400800.4950059247004114No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT3197020.46534457815387825No Hit
GCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATC2897260.42171279269510525No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC2793070.40654734124411257No Hit
TGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATATCT2401100.34949386197311155No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG2365930.344374666968491No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT2304720.3354652007690932No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG2284430.3325118750186311No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT2275510.3312135179119716No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG2249510.32742906894637214No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA2186190.318212480157843No Hit
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTA2150760.31305544066356644No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT1923550.27998372337611044No Hit
AGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTAT1820350.2649623720972694No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC1802090.262304524477583No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1671790.2433386129307518No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG1617170.235388358988404No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATATCTC1540070.2241660122481071No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1437750.2092727500111787No Hit
AGCGGATGACTTGTGGCTGGGGCTGTAGGCACCATCAATATCTCGTATGC1304690.18990510465107613No Hit
CCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCCG1155740.16822457875007454No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT1137630.16558856448980505No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG1116420.16250132746825255No Hit
CGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT1108760.16138637058069516No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1099000.1599657466613009No Hit
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATCT1061840.15455689575508258No Hit
TAGTCCGGTTCGACTCCGGAACGCGCCTCCACTGTAGGCACCATCAATAT1036960.15093546920646278No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT1020010.1484682995923508No Hit
ATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC985580.1434568158275204No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC946690.13779615350936028No Hit
AGCGGATGACTTGTGGCTGGGGGCTGTAGGCACCATCAATATCTCGTATG924180.1345196940395278No Hit
CGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTAT831570.12103977793552138No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC825590.12016935467343348No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGCTGTAGGCACCATCAATATC804890.11715635107389852No Hit
ACGCTTTAGCAGCTTAATAACCTGCTCTGTAGGCACCATCAATATCTCGT769710.11203570051198355No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC767430.11170383344884635No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA766350.11154663326104454No Hit
GGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTAT760690.11072278783237942No Hit
TATCCTGTCTGAATATGGGGGGACTGTAGGCACCATCAATATCTCGTATG747910.10886258561005784No Hit
ATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG747030.10873449656814524No Hit
TGAGGCGTGGCTTCCGGAGCTAACGCGTTCTGTAGGCACCATCAATATCT719900.10478556962827162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)