FastQCFastQC Report
Sun 2 Jul 2023
SRR5186138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5186138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43070262
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT11433502.654615846079599No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT4112180.9547608510020209No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG2888370.6706181634093612No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2663670.6184475961627538No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA2296680.5332403132351505No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2033710.47218426486469944Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1938050.449974044736482No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT1903330.44191279820865725No Hit
CGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAT1550260.35993744361248603No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1469690.3412308009642477No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1246190.2893388482289706No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC980120.22756304570424948No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC965020.22405714643667596No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATATCTC889140.20643942217022038No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA836460.19420824512281815No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT806880.1873403974185251No Hit
ACGCTTTAGCAGCTTAATAACCTGCTCTGTAGGCACCATCAATATCTCGT767890.17828774758788327No Hit
TAGTCCGGTTCGACTCCGGAACGCGCCTCCACTGTAGGCACCATCAATAT747260.1734978997805957No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC713390.16563400519829669No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG706260.16397857064347554No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC664210.1542154538089413No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT579910.13464278438798447No Hit
AGTCCGGTTCGACTCCGGAACGCGCCTCCACTGTAGGCACCATCAATATC525930.12210977495330769No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG513900.11931666447722096No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG491210.11404852842548299No Hit
TTATCCTGTCTGAATATGGGGGGACTGTAGGCACCATCAATATCTCGTAT490680.11392547368297877No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATAT480280.11151081458478242No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT469260.10895220465573206No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGT461000.10703440810274152No Hit
AGCGGATGACTTGTGGCTGGGGCTGTAGGCACCATCAATATCTCGTATGC460790.1069856505632587No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT442740.10279482395533142No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT439440.10202863404917295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)