FastQCFastQC Report
Mon 3 Jul 2023
SRR5223046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64104778
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC9628381.501975406575778No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG7437651.1602333292535543No Hit
GTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGC6850641.0686629318020568No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG6837491.0666116026484016No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC5816670.9073691823720222No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT5300490.8268478833200233No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT5127740.7998998140201031No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT5029770.7846170218388402No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA4872270.7600478703787104No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG4722040.7366127997510576No Hit
CTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTT4353970.6791958627483274No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT4257880.6642063404384615No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA4197740.6548248244460031No Hit
CCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTA3705730.5780739151768064No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT3577220.5580270475314648No Hit
GGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAA3381560.5275051416604235No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA3210420.5008082236865402No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT3141450.49004927526619No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT2999670.4679323591136998No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA2897230.45195227101480645No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC2862010.44645814076448403No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT2752780.4294188492470873No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA2713170.4232399026481302No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT2703920.42179695248301147No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT2668840.41632466147843145No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG2534460.3953621054580362No Hit
GTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATG2531280.3948660425904603No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT2480210.38689939773288035No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT2436490.38007931327677325No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG2409290.3758362598182619No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT2400350.3744416679830012No Hit
CCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC2337360.3646155673450737No Hit
CTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGG2275500.3549657406192094No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG2248830.350805364305294No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA2227200.34743120083810286No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG2211930.34504916310606365No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2183110.340553398375391No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT2114600.3298662074767656No Hit
CACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAG2112140.32948246073015025No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG2077370.3240585280554283No Hit
GTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAA2057770.321001033651501No Hit
GGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTG2055060.3205782882517743No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG2041550.31847080103763875No Hit
CTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG2041150.3184084031926606No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT1990660.310532235210299No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA1949660.304136456100043No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA1880400.2933322692420836No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA1798440.2805469508060694No Hit
GGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGG1747590.2726146247632275No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT1745530.27229327586159024No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG1732000.27018266875520575No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG1714690.26748240201377815No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1705350.2660254123335393No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA1689910.26361685551738434No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT1669890.2604938433762301No Hit
CTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTA1668160.2602239726966998No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1666920.2600305393772676No Hit
GTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTAC1601830.2498768500532051No Hit
GTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATT1590200.24806263271046658No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG1585520.24733257792422272No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC1542000.24054369239060464No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG1540180.24025978219595423No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1534580.23938621236626076No Hit
CCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGT1533820.23926765646080234No Hit
GTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG1523100.23759539421538906No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1511030.23571253924317467No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC1503040.23446614228973695No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA1469910.2292980407794252No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT1456330.22717963394241844No Hit
AGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTC1433860.22367443500077325No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG1431450.22329848798478016No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT1424870.2222720434348903No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA1393570.21738941206535337No Hit
GTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTT1361640.21240850408997597No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGG1313630.20491920274647857No Hit
GGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAA1307700.20399415469467813No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG1285100.20046867645341507No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC1276980.1992020002003595No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT1215890.189672289326078No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG1198390.1869423836082858No Hit
CTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTT1179000.18391764807297203No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC1178590.1838536902818695No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT1174960.18328742983869314No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA1165680.18183979983520104No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT1163430.1814888119571992No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC1142910.1782878025098223No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT1141410.17805381059115438No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA1130310.17632227039301188No Hit
GAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACT1119650.17465936782434532No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT1110180.17318209884448862No Hit
GGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTG1099930.17158315406692462No Hit
GGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTA1099310.17148643740720856No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT1094280.17070178450660883No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT1089720.16999044907385844No Hit
CCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAA1085760.16937271040857516No Hit
TGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAG1079730.1684320628955302No Hit
CAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGT1075110.16771136778603304No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT1072500.1673042218475509No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA1056990.16488474540852477No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA1047360.16338251729067682No Hit
CTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGGAAGATTTG1044740.16297381140607023No Hit
GCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAG1023650.15968388502959951No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG1006420.15699609785716753No Hit
GCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATT1001560.15623796404068352No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA970900.15145516922311159No Hit
TGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGAC964800.15050360208719543No Hit
CTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTAC964420.15044432413446623No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG955870.14911057019805918No Hit
GTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCAT953880.14880014091929308No Hit
GTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGA950520.148275999021477No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC941770.1469110461625809No Hit
CTTTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA941160.14681588944898927No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT935810.14598131827240707No Hit
GGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCT915710.14284582656225717No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC913740.14253851717574No Hit
CACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATT912600.1423606833175524No Hit
CTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTT903890.1410019702431541No Hit
ATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT897630.1400254439692467No Hit
TGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTA896110.13978833215832992No Hit
GCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGC891170.1390177187728503No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC890400.13889760292126743No Hit
GTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAG886960.13836098145445572No Hit
GTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGC884330.13795071562372466No Hit
TGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGA872820.1361552176344796No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT871000.1358713074398292No Hit
CTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTA868870.1355390389153208No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG858650.13394477397613014No Hit
CGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCT852440.13297604743284502No Hit
CTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACT851280.13279509368240852No Hit
CCAGGTTTCAATTTCTATCGCCTATACTTTATTTGGGTAAATGGTTTGGC848250.13232243000669935No Hit
GTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGA843760.1316220141968201No Hit
ATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG843240.13154089699834856No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC842620.13144418033863248No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTC841570.13128038599556494No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT840050.13104327418464815No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA838700.13083268145784702No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT826310.12889990820965014No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG817420.1275131161050117No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG802420.12517319691833267No Hit
CCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTA792200.12357893197914203No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG768730.11991773842505156No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT752320.1173578668348247No Hit
CTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTG730520.11395718428351784No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA726750.11336908459459918No Hit
ATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTA722500.11270610749170677No Hit
CCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTT721130.11249239487265678No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT717740.11196357313646729No Hit
CTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGG716430.111759220194164No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA712360.11112432212151174No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT705860.1101103571406175No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT697070.10873916449722358No Hit
GAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCT695800.1085410513394181No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT690740.10775171860044504No Hit
CGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGT683440.10661295792959458No Hit
CTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGG667960.10419816132894182No Hit
CTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCT647530.10101119139668496No Hit
GGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTT645450.1006867226027988No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG644220.10049484922949113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)