FastQCFastQC Report
Mon 3 Jul 2023
SRR5223059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41729373
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA18191124.359308250330049No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6719631.610287794163598Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5962821.428926334455109No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA5818031.3942289523497033No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA5750051.3779382690461224No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGAT4794741.1490083975141443No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT4070460.9754424060002052No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA4019290.9631800602419787No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3870230.9274594180938209No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA3557740.8525745162765805No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT3269890.7835943281486639No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3053530.7317459574578319No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACCTGTAGGCACCATC2896510.694117786049649No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGAT2796260.6700939407836298No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT2488330.5963017944218811No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATA2442330.5852783841252539No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT2409910.5775092762596744No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT2383340.5711420586166008No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGCCTGTAGGCACCATC2381710.5707514464691333No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC2374690.5690691782021263No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGTAGGCACCATCAAT2358990.5653068403400167No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA2357960.5650600118051139No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG2354380.5642021029168112No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1868540.4477757190360852No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1839470.44080940300732535No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCATCAATAGATC1825330.43742090253788385No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1797740.43080925275345017No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1709220.4095963771130709No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAATAGATC1647320.394762701083479No Hit
TGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT1559810.373791861190917No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC1529970.3666410228593658No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAA1443530.3459265970758775No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCCTGTAGGCACCATCAAT1435210.3439327976483136No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATC1408320.3374888954118721No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGTAGGCACCATCAATA1340270.32118143735349197No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1285120.307965326965253No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGTAGGCACCATCAAT1278070.3062758695176177No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1276630.30593078884746244No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1234930.29593782777421557No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1181350.28309795117218756No Hit
TCGGGTCTTCCCGGAGTCGGGTTGCTTCTGTAGGCACCATCAATAGATCG1127840.2702748493249587No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG1106230.2650962428790866No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA1053500.2524600597281919No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCGGGCCTGTAGGCACCATCAATAGA1021840.2448730777718611No Hit
TGGGGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC1013480.24286969277012624No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA990730.2374178974603812No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAATAGATCGG983900.23578116067068633No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAATA981350.2351700803172863No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG963600.23091648177891388No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA947070.22695524325275626No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA902620.2163032739552545No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG893870.21420642960535258No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGCCTGTAGGCACCA882500.2114817301472514No Hit
TGCGGGCGGGCGCGACCCGCTCCGGGGACCTGTAGGCACCATCAATAGAT875760.20986656089944125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA869530.2083736077223111No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGCTGTAG858310.2056848541673511No Hit
GCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCCTGTAGGCACCATCAA846380.2028259566708563No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGTAGGCACCATCAATAG826980.19817695319793086No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAAT821760.19692603576861792No Hit
GCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA820430.19660731542743287No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA805950.193137337577538No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAG781750.18733806520409496No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC767140.18383693423814446No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAA759060.18190064825560642No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA758950.18187428792663624No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT715170.17138287699649837No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT710750.17032367105060506No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATC689640.16526488428187022No Hit
GCGTCTCTCGGGGTCGGGGGTGCCTGTAGGCACCATCAATAGATCGGAAG688220.16492459639880044No Hit
AGATAACCTCGGGCCGATCTGTAGGCACCATCAATAGATCGGAAGAGCAC687620.16478081278623574No Hit
TGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG680020.16295955369374948No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA678480.16259050908816675No Hit
GCGGGGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGAT673910.16149535723913228No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGTAGGCACCATCAATA668550.1602108903002209No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA656210.1572537406684735No Hit
GAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGATCGGAAGA642070.153865240199032No Hit
AGAAAAGGTGTTGGTTGATCTGTAGGCACCATCAATAGATCGGAAGAGCA608540.1458301326502078No Hit
GCGCCGGGTTTCGCCCCCGCGGCGTCTGTAGGCACCATCAATAGATCGGA600650.14393937814498195No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG585500.1403088419277232No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA578350.13859542054466048No Hit
GCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA571810.1370281791677052No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG571370.13692273785182443No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT559690.13412375019389822No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA553430.13262360783613977No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCTGTAGGCACCATC540910.12962332312062297No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA538060.12894035096094061No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA528210.12657990332133676No Hit
GCGGGCGGGCGCGACCCGCTCCGGGGACCTGTAGGCACCATCAATAGATC520990.12484970718347481No Hit
CCCCCCGCCTCCCCTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA512350.12277922316254308No Hit
AAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC500530.11994668599501844No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT487490.11682178881527888No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAG487280.11677146455088122No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG481040.1152761149802083No Hit
TGGCCGCGGGTGCGCGTCTCTCGGGGTCTGTAGGCACCATCAATAGATCG479570.11492384512942477No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA470190.1126760279863299No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT453840.10875792454394174No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATAGATC445010.10664190904569786No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC441860.10588704507973318No Hit
CTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAGATCGGA440630.1055922886739755No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAAT439690.10536702768095749No Hit
TGGGGGCCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA438520.10508664963645631No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATC425840.10204802262425558No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGTAGGCACCATCAATAGA423320.10144413145148383No Hit
TGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCTGTAGGCACCATCAATA420160.1006868710919764No Hit
TCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG418580.10030824091222268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)