FastQCFastQC Report
Sun 2 Jul 2023
SRR5223061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30013323
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1267887542.24415603697065Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG4108991.3690553358586786No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3633991.2107922871452788No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA3212701.0704246244242932No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG2707400.9020660591298071No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2418230.8057188469267464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1901640.6335986188533672No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1775440.5915506257004597No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG1676220.5584919737144733No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1661740.5536674496189575Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGTCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1598110.5324668647986762Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA1585340.5282120876785287Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1090790.3634352650654511No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1063970.354499233557044No Hit
GTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1001410.33365515707807497Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA903900.30116625206745684No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA900930.30017669153129095No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA876250.2919536767055084No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA873660.29109072660831325No Hit
GTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGAA838630.2794192432474072No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG797900.26584860330194027No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG790640.2634296775468681No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG790500.2633830315956684No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA772900.25751896915912975No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT652960.21755671639558205No Hit
AGAAAAGGTGTTGGTTGATCTGTAGGCACCATCAATAGATCGGAAGAGCA625090.2082708402531769No Hit
TGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA620500.20674151942455687No Hit
CCGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA570480.19007558743162162Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA557240.18566421318958917Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGA548080.18261223523966338No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGA537920.1792270719240252No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG516120.17196363095149444No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACTTCTGAACTCCAGTCA511480.17041765085458882Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG489290.1630242675894302No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTTAACTCCAGTCA457550.15244896408171796Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA447710.14917042008310777No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCATTCA447470.14909045559533676Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGAACACGTCTGAACTCCAGTCA444190.14799760759580002Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGA434650.14481901920690354No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT431800.14386944091462317No Hit
CTGTAGGCACCATCAATAGATCTGAAGAGCACACGTCTGAACTCCAGTCA401970.13393052145542164Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC386950.12892607726242108Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC384960.12826303838465336Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA384190.1280064856530548No Hit
TCGCCGCGACCGGCTCCGGGACGGCCTGTAGGCACCATCAATAGATCGGA361240.12035988150995477No Hit
CGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGAAGA355810.11855068497413632No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC347210.11568529082900951Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATA344860.11490230521958532No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT319590.10648271102803246No Hit
CTGTAGGCACCATCAATAGATCGAAGAGCACACGTCTGAACTCCAGTCAC313980.10461354112638577Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
TAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG311290.10371727249261936No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG310540.10346738346833505No Hit
CTGCAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA310200.10335410044399282Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA300230.10003224234783999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)