FastQCFastQC Report
Mon 3 Jul 2023
SRR5223062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27421921
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA26798109.772510102410404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG13494944.921223425594436No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG8328663.037227041825407No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA4211081.5356619253625594No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3931641.4337580507215377No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3531721.2879185232865342No Hit
GTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGAA3317611.2098386542649582No Hit
AGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGA2533910.924045401487372No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA2520720.9192353810661186No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2447460.8925195284458737No Hit
TGTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGA2446400.8921329763877593No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG2171140.7917534296740188No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2162850.7887303008421619No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2132290.7775859320723738No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2107620.7685894799273909No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1939370.7072334574955562No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA1761870.6425042213490441No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1750110.638215681534492No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGG1584390.5777822786375907No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1248960.455460432549565No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG1160580.423230743024896No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGATCGGA1105390.40310450898024247No Hit
TAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG1057680.3857060196475659No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1033450.37687002307387585No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGG968980.3533596351619568No Hit
GGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG912230.3326645131827198No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA878620.32040789556647037No Hit
ACGTACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG873720.31862100397707366No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATAG869220.3169799810888522No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG868070.3165606085729734No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA804640.29342947928410995No Hit
CGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGAAGA789700.28798128329521483No Hit
AGAAAAGGTGTTGGTTGATCTGTAGGCACCATCAATAGATCGGAAGAGCA684100.2494719461849518No Hit
AGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGATCGG647740.2362124812481226No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAGATCG624230.22763904833654797No Hit
ATGTACACGTAGGGATAACAGGGTAATAACGCGACTGTAGGCACCATCAA620620.22632258330844146No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC581890.212198846317149Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT572320.20870893764153137No Hit
TGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAATA566990.20676523719837134No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA565010.20604318712755387No Hit
TCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATAGAT564970.20602860025743636No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGCTGTAGGCACCATCAAT496240.18096471067800102No Hit
TCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGAAG486680.1774784487199128No Hit
AGAAAAGTTACCACAGGGATCTGTAGGCACCATCAATAGATCGGAAGAGC480890.17536699927040122No Hit
TGGCCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATCGGA452230.16491550683119538No Hit
TTGTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGG442450.16134901708746077No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT432320.15765489223019788No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG422210.15396806080799372No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG409440.14931120252297422No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCCTGTAGGCACCATCAATAGA406150.1481114324558079No Hit
GTCGCGGCGTGGGAAATGTGGCGTACCTGTAGGCACCATCAATAGATCGG404780.1476118321542827No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTCTGTAGGCA402120.14664180529146736No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA396270.14450847553677948Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG390400.14236785234703286No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG374960.13673732048166867No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT368930.13453834981145193No Hit
CCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAAG366430.1336266704291067No Hit
TACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGA359250.13100832724301117No Hit
TTCGCGGCGTGGGAAATGTGGCGTCTGTAGGCACCATCAATAGATCGGAA355220.12953870007867066No Hit
CTGTAGTCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA340850.12429836698895019Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA335750.12243854104896588No Hit
CTGTAGGCACCATCAATATATCGGAAGAGCACACGTCTGAACTCCAGTCA333650.1216727303677959Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTCGCGGCGTGGGAAATGTGGCGTACTGTAGGCACCATCAATAGATCGGA330760.12061882900180479No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCCGCCTGTAGGCACCATCAATAG327770.11952846046051988No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATCGG325190.1185876073379396No Hit
GCGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG317610.11582339545066883No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA317300.11571034720725804No Hit
GAGGGCTCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG315370.1150065307240875No Hit
AAGTCTGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG309810.11297895577775169No Hit
TCGCCGCGACCGGCTCCGGGACGGCCTGTAGGCACCATCAATAGATCGGA306880.11191046754164306No Hit
TGTCGCGGCGTGGGAAATGTGGCGTACCTGTAGGCACCATCAATAGATCG297890.10863206848272955No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAATA293000.10684882361086227No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA281480.1026478050170154No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCGG279940.10208621051749074No Hit
CCCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA276900.10097760838855892No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA274930.10025920503527086No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA274820.10021909114244767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)