FastQCFastQC Report
Mon 3 Jul 2023
SRR5223063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81866425
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA6704920.8190073036656969No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC4692690.5732130113169105No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA4035010.49287726928347No Hit
CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC3438470.42000979033834684No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG2846940.34775428388377777No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG2810480.3433006876750756No Hit
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT2105160.25714571022247523No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG1983410.2422739236506297No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1383720.16902167158270315No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA1232470.1505464541782544No Hit
GGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGA1105300.1350126135347916No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1104180.13487580531335036No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1048770.12810746285794206No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1015240.12401176672854593No Hit
CCCCGACCCGCCCCCCGACGGGAAGAAGGAGGGGGGAAGAGAGGTGGCGA939590.11477110427137865No Hit
GTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCAT925700.11307443802511224No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA858220.10483174268327951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)