FastQCFastQC Report
Mon 3 Jul 2023
SRR5223064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52505555
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC8083531.5395571001963506No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA7786041.4828983333287307No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG6007161.1440998957157962No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA5735721.0924025086488467No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA4523150.8614612301498384No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG3169930.603732309847977No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC2988940.569261671455525No Hit
GGCGGACGGGGGGAGAGGGAGAGCGCGGCGACGGGTATCTGGCTTCCTCG2557600.4871103638462635No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG2248840.42830515742572384No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1486330.28308052357507696No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1465940.2791971249518265No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA1338250.25487779340681194No Hit
CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC1318500.25111628664814606No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1305970.24872987248682546No Hit
GGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGA1283350.244421756897913No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT1173230.22344873794782286No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC1153290.2196510445418585No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1095980.20873600898038314No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC930180.17715839781143156No Hit
CGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG906240.17259888025181336No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA881010.16779367440264178No Hit
GTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCA856490.16312369234074375No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT845970.16112009481663417No Hit
TGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC816550.1555168781665102No Hit
GACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGG814110.15505216543278136No Hit
GGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGCACCCGC810970.15445413347216308No Hit
GAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAG794300.15127923131181073No Hit
GGCGGACGGGGGGAGAGAGAGAGCGCGGCGACGGGTATCTGGCTTCCTCG791640.15077261824963092No Hit
GGCGATGGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGC774350.1474796333454622No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC761220.1449789455610935No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA746840.14224018772870795No Hit
ACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGC727950.1386424731630777No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC702950.13388107220274123No Hit
GTACTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTT690270.13146608963565856No Hit
GGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGCACCCGCC684410.13035001725055567No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC673020.12818072297302638No Hit
ACCGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTG665120.12667612026956004No Hit
GGTACTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGT664860.12662660169957254No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG654120.124581103847012No Hit
GGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGA647260.12327457542349568No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC647130.12324981613850192No Hit
GGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCT641660.12220802160838029No Hit
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT639240.12174711799541973No Hit
GCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGG631490.12027108369771541No Hit
GCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACT630480.12007872309891782No Hit
GTCCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGA628560.11971304750516398No Hit
AGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGG620200.11812083502402745No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA614580.11705047208814383No Hit
GCGGACGGGGGGAGAGGGAGAGCGCGGCGACGGGTATCTGGCTTCCTCGG601130.11448883837148278No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC600600.11438789667112366No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT589960.11236144442240445No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGG586550.11171198933141455No Hit
GTGATTTCGTCATTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCAT564820.10757337961669008No Hit
CCGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTGC559030.10647063915427614No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC556030.10589927103903578No Hit
CCCCGAGCCACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTC552110.10515268336845501No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC542490.10332049627891754No Hit
GCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGAT536020.10208824571038246No Hit
GCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTC534200.10174161572046996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)