FastQCFastQC Report
Mon 3 Jul 2023
SRR5223065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5223065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70833857
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA12117391.710677706001524No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA9604091.3558615056074104No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC7165771.0116306387212546No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC6745240.9522621364526288No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA6077710.858023303743011No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA5684540.8025173611540029No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG5176380.7307776562273038No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA5104360.7206102019829302No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC4892730.6907332463909173No Hit
CGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG4770980.6735451381674726No Hit
GTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGG4446350.6277153593372728No Hit
GGCGGACGGGGGGAGAGGGAGAGCGCGGCGACGGGTATCTGGCTTCCTCG4290520.6057159925655327No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC4285110.6049522335060761No Hit
CCGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTGC4275240.6035588320427052No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT4136550.5839792120878015No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA4101930.579091718808987No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG4086780.5769529110916549No Hit
ACCGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTG3592940.5072348382779721No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC3474960.49057896141389No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC3118890.4403106271623752No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT3046240.43005423239906304No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC2915150.41154754568849755No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA2895770.40881156591543505No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG2842290.4012615040855392No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA2681280.37853084860252634No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC2661220.37569886953918097No Hit
TGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC2465650.34808919130296684No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG2424520.3422826459951207No Hit
GTCCAGCCGCGGCGCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGA2414400.34085395067502816No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGG2257730.3187359965447032No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT2196940.31015394234426624No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1913960.27020412004389366No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC1889870.2668032040101953No Hit
GTACTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTT1877660.2650794520479098No Hit
CCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCA1874870.2646855726068961No Hit
CACCGGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACT1853940.26173077092215946No Hit
GGTACTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGT1846750.26071571960284473No Hit
GGCGGACGGGGGGAGAGAGAGAGCGCGGCGACGGGTATCTGGCTTCCTCG1832310.25867714643860207No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC1831550.25856985311416825No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1798930.25396471068912707No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG1765250.24920992231158612No Hit
GGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGA1701920.2402692825268572No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1683050.23760530222150686No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT1636580.2310448801340862No Hit
CTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTAC1609510.22722326132826567No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC1608780.22712020326663843No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC1605870.2267093827743984No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC1541960.21768686124207523No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1526260.2154704070399555No Hit
GCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACT1472490.20787940433626254No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACG1469860.20750811296355076No Hit
ATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTT1464390.20673588337848098No Hit
ACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGC1445720.20410013815850803No Hit
GACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGG1413210.19951052503042435No Hit
GTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAAC1385750.19563384780811807No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1365780.1928145745331925No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC1356430.19149458429180272No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC1343350.18964800970812587No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG1317240.18596191931211653No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC1242550.1754175266779557No Hit
CCCCGACCCGCCCCCCGACGGGAAGAAGGAGGGGGGAAGAGAGGTGGCGA1193660.1685154600574694No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT1128460.15931082222446252No Hit
GGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCT1116380.15760542306767228No Hit
GTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCT1111760.15695319259545615No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1099480.1552195583532886No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC1098690.15510802976604818No Hit
GAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAG1065220.15038288822815338No Hit
ACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGC1041950.147097736044502No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1023240.14445634380745354No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC1010020.14259000466401256No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG1005090.1418940098094616No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG1004840.14185871595273997No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG997760.14085919393038274No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC992800.14015896381302517No Hit
GGCACGGCCGGACGCGCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGC987580.13942202808467707No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG984540.1389928547869418No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT973140.13738345492043447No Hit
GCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGAT965630.13632322746451603No Hit
CTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGG961360.13572040839171018No Hit
GTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCACGCCCG952810.1345133584918297No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG933110.1317322025821635No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC924720.13054774075058484No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG918360.129649865035586No Hit
AGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGG916570.12939716102145898No Hit
GGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGCACCCGC915060.12918398612686022No Hit
CTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTG903250.12751670433532936No Hit
GCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCG894920.12634071302936392No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC891670.12588189289198243No Hit
GCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTC888880.1254880134509688No Hit
GGCGGACGGGGGGAGAGAGAGAGAGCGCGGCGACGGGTATCTGGCTTCCT886850.12520142733438896No Hit
CGGGGGGAGAGGGAGAGCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCG881670.12447013862311633No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA876120.12368661500389566No Hit
GGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGG874840.1235059104574808No Hit
CCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCC865790.122228272844157No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG852850.12040146282024428No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT849210.11988758426637702No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG835440.11794359863814842No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG831150.11733795605680487No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA828150.11691442977614505No Hit
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT814060.11492526801131273No Hit
AGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACT811780.11460338803801126No Hit
GCGGACGGGGGGAGAGGGAGAGCGCGGCGACGGGTATCTGGCTTCCTCGG807760.11403586282192708No Hit
CCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCG803120.11338080884117321No Hit
CCTTACGGTACTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGA801480.11314928114107918No Hit
GGGAGAGGGAGAGCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGAT797950.11265093188416946No Hit
CCCCGAGCCACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTC797630.11260575574756573No Hit
GGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGA792610.11189705510459497No Hit
GCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCT790500.11159917495386423No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC789180.11141282339037391No Hit
GACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCC785630.11091165062492643No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC781560.11033706663749795No Hit
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC762680.10767167457787875No Hit
GGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGCACCCGCC756290.10676956360007334No Hit
CGGGGGGAGAGAGAGAGCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCG752520.10623733224071083No Hit
GCCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCT749540.10581662946858873No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA741990.10475075499559482No Hit
CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC727400.10269100551731922No Hit
CTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCG727400.10269100551731922No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT726720.10259500622703632No Hit
GGCGATGGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCCGGC722850.10204865732498514No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA715630.10102937074286382No Hit
CCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA715610.10102654723432608No Hit
CGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG709120.100110318713832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)