FastQCFastQC Report
Mon 3 Jul 2023
SRR5227288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42082855
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG12228002.905696393459997No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG11057082.627454815030967No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG9524532.263280378672027No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT8619842.0483020935723113No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT7892971.875578546179911No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG6714341.5955048677186945No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA5804211.3792338946585254No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC4709291.11905192744171No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4651781.1053860295362565No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA4147000.9854369433822872No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3882500.922584743834514No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3547670.843020275121543No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2988150.7100635163655128No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT2855270.6784877119197354No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2669010.6342274068620106No Hit
TTTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2551870.6063918429488684No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2551260.606246890806244No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2535540.6025114028028754No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2270420.5395118748478448No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG2130200.5061918921613089No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1938610.4606650380540959No Hit
GCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA1526490.36273441999122924No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATC1505140.3576610949993768No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1416530.33660501408471455No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGG1394910.3314675299477661No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC1391020.3305431630054567No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA1317420.31305385530520685No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1252050.2975202133980691No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1210020.28753277314478787No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG1208250.2871121743047139No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1204500.28622107506726907No Hit
TTTGGTGACTCTAGATAACCTCGCTGTAGGCACCATCAATAGATCGGAAG1178620.2800713021965834No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1074430.2553130009834171No Hit
GCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAAG1066150.253345453867139No Hit
TAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATCGGAAGA1058120.25143731336669056No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1051810.2499378903831501No Hit
TCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA1047860.24899926585304158No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA1010540.24013104624199094No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG995550.23656902555684495No Hit
TGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCG992860.23592981037051786No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA968240.23007944684361364No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT952150.22625603704881717No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA941520.2237300677437403No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGAT932520.22159142957387276No Hit
AACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAG929490.22087142139001736No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC882090.20960792702871514No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT870650.20688948028835022No Hit
TCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA852380.20254804480351915No Hit
CGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG843010.20032148484222376No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCG838590.19927117587435547No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA798880.18983502901597338No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG796860.18935502356006978No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA778880.18508249974960111No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA760370.1806840339135736No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC727040.17276394389116423No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA706370.1678522048943685No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG706280.16783081851266984No Hit
ACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCGG700040.16634802938156168No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG696340.16546881146728282No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG683370.16238679623804042No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG679060.16136262618113717No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA661910.157287332335223No Hit
CACTCGCCGAATCCCGGGGCCGAGGGCTGTAGGCACCATCAATAGATCGG639010.15184568632522674No Hit
CGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAAG634850.1508571602378213No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA610840.14515174885354143No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT595490.14150418264160072No Hit
TGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA594240.14120714956245245No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG588540.13985267872153637No Hit
TAACTGTGGTAATTCTAGAGCTACTGTAGGCACCATCAATAGATCGGAAG587930.139707726578912No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA585890.13922296859374203No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG578470.13745978023591793No Hit
ATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCA558190.13264071555981646No Hit
GCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA555520.13200625290275578No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA551890.1311436688409092No Hit
TCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA550650.13084901202639412No Hit
TTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATAGATCGG546390.12983672329265683No Hit
CGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAAG540250.12837769680788055No Hit
TTAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATCGGAAG535080.12714916799252332No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATC533120.12668342012441883No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG516620.12276258347966172No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA515280.12244416401881479No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCCCCATCAATAGATCGGAAGAGC481310.11437199305988151No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA478380.11367574752235798No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA477440.11345237864683849No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA477020.11335257553224465No Hit
TGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG473080.11241632726676934No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCGG458050.10884480152309059No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGCTGTAGGCACCATCAATAGATCG457370.10868321552803392No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG455220.1081723186318989No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC454240.10793944469784666No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC447370.1063069508948478No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAAATCGGA433610.10303721075958369No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG433480.10300631931935227No Hit
ATTGATCATCGACACTTCGAACGCACTGTCGGCACCATCAATAGATCGGA428770.1018870986771216No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATCG427230.10152115392361094No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA425780.10117659555179893No Hit
TCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA424980.10098649438114406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)