FastQCFastQC Report
Tue 4 Jul 2023
SRR5227289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41657461
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG24979375.99637361480096No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT12420522.9815835391408037No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA9429912.26367852807928No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC7514021.8037633162520395No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT7207811.7302566759889664No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG5846551.4034820797167642No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4823141.1578094017779912No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG4172771.0016861085220725No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA3831770.919828023124117No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA3520140.8450202954039855No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3332750.8000367569209271Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG2870970.6891850657916958No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2604850.6253021517562004No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2366550.5680975131921746No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG2178090.5228571179602136No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2145910.5151322112502248No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2117390.5082858986533049No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1951780.468530715302116No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1917940.4604073205517734No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1912520.459106233094715No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1586670.38088495119757776No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1556310.37359694101375984No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1485070.35649556270364147No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA1319350.31671397351845326No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA1311390.31480315134904646No Hit
GCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA1269650.3047833376114785No Hit
TTTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1227960.29477552652572847No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA1215500.29178446569271227No Hit
GCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA1081830.2596965763227865No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA1067710.2563070274494165No Hit
ACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1063810.2553708206076218No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG990210.23770291713169941No Hit
CGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAAG985930.23667549013608868No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA958010.2299732093609834No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT884950.21243493452469414No Hit
CGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAG876740.21046409909619793No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA864130.20743703030772806No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC802280.192589750009008No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG767430.1842239016919442No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG758510.18208262860763405No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG757950.18194819890727376No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC757730.1818953872392751No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA757640.18187378246600291No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG755490.18135766843783396No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG719290.1726677485216874No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCG715650.1717939554693456No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG715610.17178435334789127No Hit
TCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA710910.17065610407701035No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA708930.17018079906502223No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA697020.16732176740200275No Hit
CCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGG691690.1660422847182165No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT678290.16282557403102413No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG617730.1482879621492054No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA601760.14445431515857388No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG585350.140515044831945No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGAT583850.14015496527740853No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC567760.1362925119224141No Hit
TCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA549720.13196195514652226No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT545030.13083610640600493No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG541900.13008474040220552No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC539310.1294630030380392No Hit
TCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA533840.12814991292916292No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG498310.11962082854737595No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG497340.11938797710210904No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA497160.11934476755556467No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG479030.11499260600640064No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAAATCGG478950.11497340176349201No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA473890.11375873339952236No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG461510.11078687680941476No Hit
AACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAG458900.11016033838452133No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCGG450710.10819430401675224No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA445270.10688841549896667No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG441530.10599061714298912No Hit
AAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCGGA427810.10269708948416227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)