FastQCFastQC Report
Mon 3 Jul 2023
SRR5227290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40462973
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG25769786.368731234850193No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT10919712.6986919621551286No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA10158392.510539697614409No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT7496191.8526048493767378No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG7307981.8060907190383662No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG6560091.6212575383425236No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT4636231.1457956883197882No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA3758560.9288887398362943No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2823420.697778682747805No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA2742980.6778987792122937No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2466010.609448544475464No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2305000.5696566092659578No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA2228320.5507059503512013No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1958760.48408701950793387No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1798980.4445990659163873No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1731130.4278306490232441No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1688020.4171764640230465No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1666770.41192474907862064No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1522640.37630452908143946No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1422180.351476892219462No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1289840.31877044724321174No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1224670.3026643642818831No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1174010.29014427585437186No Hit
CTAAACCATTCGTAGACTACCTGCTTCTGTAGGCACCATCAATAGATCGG1133810.28020926687715214No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT1048130.25903435222122706No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG960310.23733055897795746No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA952060.23529165788188622No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG932720.23051197943364168No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC902350.22300635200483168No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA827550.20452031540045262No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG748840.1850679632462993No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG746430.18447235698671968No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG704330.17406778290858657No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT692130.17105268068166912No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA646630.1598078322124279No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA625140.15449680378157088Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG624450.15432627750808128No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG614270.1518103971252928No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA608710.15043630135630418No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG567510.14025415285228796No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATATCGG561290.13871694499561366No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCCGCTCTGTAGGCACCATCAAT561010.13864774592811063No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA541780.13389525282781373No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA510630.1261968565681024No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG493630.12199548461256171No Hit
CTAAACCATTCGTATACGACCTGCTTCTGTAGGCACCATCAATAGATCGG491570.1214863771873609No Hit
GCGCCGCTACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT488620.12075731558331121No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG480620.1187801993689391No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGT461350.11401782068757034No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTCGGCACCATCAATAGATCGGA459370.11352848442451324No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA437270.10806670088231035No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCTG437090.10802221576748697No Hit
TGCGCCGCTACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA436680.10792088856150042No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA434130.10729068276816932No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA432560.1069026737110988No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA426630.10543713631719547No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGTCACCATCAAT416960.10304729709307323No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG412270.10188821271239759No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC408580.10097626785851846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)