FastQCFastQC Report
Mon 3 Jul 2023
SRR5227291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41143319
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG12755613.1002870721246385No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT8537182.074985734621944No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG7277981.768933614713971No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG5858401.423900682392687No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG5440591.3223507806941874No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4821171.1717989985202701No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC4097080.9958068769318293No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3817990.9279732634112479No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT3721060.904414152878624No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3130520.7608817363518972No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2998020.7286772367586583No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2942940.7152898870409555No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2616290.6358966810626046No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG2007280.4878750788189936No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1930570.4692304964507117No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1923270.4674562108127446No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1731150.42076090166668373No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1710660.41578074923901986No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1587280.38579289142910417No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT1546200.3758082812910645No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1445810.35140820797660977No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1426840.3467974958461664No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1376460.33455249441592205No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA1318050.32035577878391386No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1312450.31899468295204864No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1234930.3001532277938005No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1178490.2864353262312163No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1169070.2841457685997573No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG1148260.2790878392674155No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1118760.2719177808674113No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1105800.2687678162279519No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1076630.2616779652608969No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1075950.2615126893384561No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1064260.25867140178943754No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1031670.25075031015363636No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1004180.24406878793614095No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA999580.2429507449313946No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA954310.23194774344772723No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT951660.2313036534558624No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG951550.23127691764487937No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA936430.22760195889884333No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT932060.22653981804433426No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGAT922930.22432074573273975No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG900180.21879129391578742No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATC879290.213713920357276No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG771040.18740345182166757No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT770260.1872138706165149No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT769720.18708262208987075No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG762090.18522812901895447No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT744130.1808629002439011No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC742790.18053720945556193No Hit
ACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCAATAGATC737240.17918826626505266No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG672790.163523511557247No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT647190.1573013591830061No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG643570.1564215079488361No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG627810.15259099539344406No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATCG627740.15257398169554576No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA599830.14579037729066047No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG590440.14350811124401508No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC588310.1429904087222521No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGA571220.13883663590679207No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC559560.13600263994258702No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA542180.13177838180726256No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAG541540.13162282799790653No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT540550.13138220569905895No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA535720.13020826054407522No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT535460.13014506680902432No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG525500.12772426065092124No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC525350.12768780272685343No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC502390.12210730981620611No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA498400.12113752903600218No Hit
CCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCGGA498130.12107190477268011No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG496680.12071947817335786No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA487970.11860248804915324No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG483480.11751118085538992No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG483100.11741882078108477No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAATAGATC482670.1173143080654237No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGAT481330.11698861727708454No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC474750.11538932967464294No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT474210.11525808114799879No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG462760.1124751262774887No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC458210.11136923591409822No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG456050.11084424180752164No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA453160.11014181913714836No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA441150.10722275468345176No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA438450.10656651205023106No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC435520.105854367266773No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT434030.10549221855436601No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA433460.10535367844290833No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA433140.1052759015382303No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA432130.10503041818284031No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC426060.10355508752222932No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA425110.10332418733646648No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC412270.10020338903626126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)