FastQCFastQC Report
Mon 3 Jul 2023
SRR5227292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60552437
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT39819076.575964894691191No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA8518401.4067807047964065No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA7536761.2446666679988454No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA7438611.228457576364763No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5354260.884235262075414No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA4934640.8149366473887748No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA4534970.7489326977872088No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3230790.5335524315891695No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2978300.49185468786334724Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA2698420.44563359192298074No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2527530.41741177155264614No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2397720.3959741537735302No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1480370.24447736100200226No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG1474410.2434930901294691No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1448730.23925213777935975No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1395060.23038874554297462No Hit
ACGAGACTCTGGCATGCTAACTAGTTACCTGTAGGCACCATCAATAGATC1345360.22218098340121306No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT1322540.21841234895302397No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1290920.21319042865277246No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG1265730.20903039790124384No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1201460.19841645679760175No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT1187260.1960713818999556No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1163040.1920715428843929No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1147710.1895398528716524No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1133670.18722120135313464No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1076320.17775007139679613No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG1023380.16900723582768437No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCTGTAGGCACCATCAATAGAT1003780.16577037188445445No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG986440.1629067381714133No Hit
AACGAGACTCTGGCATGCTAACTAGTTACCTGTAGGCACCATCAATAGAT906230.14966036792210363No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG857270.14157481390881096No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC855920.14135186664741503No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA828980.13690283018666943No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT822270.13579469972447186No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA820450.13549413378688624No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT819070.13526623214190373No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA794780.13125483289797238No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG788230.1301731258149032No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGCTGTAGGCACCATCAATAGA731670.1208324612930112No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGA724140.11958891101278055No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG697900.11525547683572174No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGCTGTAGGCACCATCAATAGAT686570.11338437130119139No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG671550.11090387658551216No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGAT663590.10958931347387389No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT659770.10895845529718318No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATCG644120.10637391852618583No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGA624430.1031221914321962No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA622250.10276217289157165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)