FastQCFastQC Report
Mon 3 Jul 2023
SRR5227293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42709138
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG35550028.32375029437494No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT13121283.0722418232838136No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG12126292.8392729443520963No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT9882052.313802259366602No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG9560932.238614602804674No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA6556651.5351866853412026No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT4756351.1136609687603622No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4079070.9550813224092699No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC3984200.932868277510073No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA2845650.6662859831074089No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2756550.6454239371443179No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2721180.6371423370801818No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2719030.6366389319306796No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2662570.6234192785628219No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2661820.6232436721153211Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA2647500.6198907596777065No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA2526570.5915759760826829No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1836830.4300789212837777No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1672270.3915485252828095No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1238490.28998243888696607No Hit
CTAAACCATTCGTAGACTACCTGCTTCTGTAGGCACCATCAATAGATCGG1231480.28834110395765894No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1214570.28438176392134157No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1193040.2793406881684196No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC1190120.2786569937328166No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1080200.25292011278710425No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1074310.2515410168193982No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG1062620.2488038976576863No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT1020860.2390261306608436No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG972020.22759063879959365No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG927220.21710108033554787No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA925910.21679435440724654No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC918010.21494463316023846No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG908150.21263599373042835No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG876460.20521603596869598No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG853640.19987291712607264No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG839490.19655980881655818No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG834570.19540783052095315No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA765100.17914199064378214No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA738240.1728529384039547No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG723440.16938763783993954No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG707470.16564839121782324No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG701740.16430675795891736No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG698180.1634732126881137No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG697220.16324843643531273No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT694720.16266308161031018No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATATCGG651740.15259966145886625No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG613940.14374909650482762No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGT581670.13619333642369463No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG572070.1339455738956848No Hit
CTAAACCATTCGTATACGACCTGCTTCTGTAGGCACCATCAATAGATCGG555040.12995813682776738No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGTCACCATCAAT553550.12960926535206588No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCG535780.12544856325594772No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCTG526420.12325699479113814No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG524730.1228612949294364No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG514720.12051753421012618No Hit
GCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA509320.11925316778812066No Hit
GCGCCGCTACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT507850.11890897915101915No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA504040.11801689839771527No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC501870.11750881040961306No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA499510.11695623545481063No Hit
TCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG494250.11572464890300525No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA490190.1147740326672011No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA473090.11077020566418364No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA469700.10997646452148016No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG469260.10987344207227971No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTATGCACCATCAATAGATCGG457160.10704032471926733No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGAT451590.10573615416916164No Hit
TCTAAACCATTCGTAGACTACCTGCTTCTGTAGGCACCATCAATAGATCG434060.10163164613624372No Hit
TTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT432930.10136706575534257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)