FastQCFastQC Report
Tue 4 Jul 2023
SRR5227296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52737784
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG48394469.176430317967094No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT31231435.922021676147788No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT19278283.65549678765418No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA16922453.2087904944963177No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG12942792.4541778243848853No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG11735132.2251845090798654No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC8462561.6046483864395973No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT8066981.5296395464777208No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6197931.1752351975957123No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA5733761.087220502097699No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA4818800.9137281915372099No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG4608040.8737644342431983No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA3993380.7572142204534039No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA3047210.577803951717046No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2864830.5432215354365287No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2803270.5315486900245942No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC2691220.5103020635072569No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2677590.5077175787287536No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2573920.4880599457876349No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA2524930.47877059074002803No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT2359110.44732823813757516No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG1855880.35190708809456234No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG1807920.34281303893997517No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1807160.3426689297373587No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1785540.3385694021576637No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC1758550.3334516292910601No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1472330.27917934511620734No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1427290.2706389786874625No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT1320520.2503935318935661No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1269760.24076855409776035No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1186780.2250341045805034No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1184320.22456764584571853No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1117710.21193723270587173No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1050060.19910961749928666No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG1049420.19898826238129386No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGTCACCATCAAT995710.1888039133384899No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA991240.1879563236862588No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG971310.18417724946501354No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC935150.17732068529841907No Hit
TCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG923400.1750926811790196No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG910340.17261627830247855No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG895140.169734094250149No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG873530.1656364628441726No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGA857960.16268412036425345No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA798750.1514568757761987No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC794750.15069840628874356No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTCGGCACCATCAATAGATCGGA779140.14773847911394988No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA773860.14673729939050908No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG756730.14348915381048244No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC740910.14048940698759735No Hit
ACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCAATAGATC724950.13746311373265133No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG706970.13405379338654047No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATAGATCGG695470.13187319361010694No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG694000.13159445607346718No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG689300.13070325442570738No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG689170.1306786041673651No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG681440.12921286188285802No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA668630.12678386334928293No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG661510.12543378766161278No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGAT660800.1252991593275895No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG659270.12500904474863792No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA644970.12229751633098576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA629130.11929397716066341No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA620100.11758173229273342No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA617210.11703373808804708No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA606660.11503327481488415No Hit
CGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCGG604430.11461042807562791No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG588560.11160120038414964No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGCTGTAGGCACCATCAATAGAT585260.11097546305699912No Hit
GCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC560780.10633362979377366No Hit
ACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG546880.10369794832486703No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT545940.1035197079953151No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA534470.10134479674003746No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA534030.1012613650964174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)