FastQCFastQC Report
Mon 3 Jul 2023
SRR5227298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49334392
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1195864224.23997036387922No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA26566215.384927009944705No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT22786674.618820477203814No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG17517893.5508474493817617No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG16079963.259381406788189No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT15341503.109696781101508No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA9658271.957715420917724No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG5019511.0174464093932687No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4970711.0075547297714746No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG4070690.8251221581893622No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG4048200.8205634722325148No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3881070.7866864965113992No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3547020.7189751117232782No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA2450130.496637315404637No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT2309280.468087252397881No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2308510.4679311746661437No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2058320.41721807375268755No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA2020520.40955607601285526No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1648960.33424147600724463No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG1571290.3184978949370654No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1505030.3050671020735393No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1487870.30158879833767893No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1303680.2642537887159935No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1265390.25649246878323745No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1197280.242686683966836No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1186600.240521865557804No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC1176310.23843609950640518No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1147230.23254163140390988No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT1138320.23073558907952085No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA1129710.22899035626100347No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG1101490.22327020874200698No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1014520.2056415329898056No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1010160.2047577681711371No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1001250.20295172584674803No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGAT969110.19643700078436155No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG927760.1880554238917143No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT887980.17999208341312892No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC880540.17848400766751113No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA776230.15734054247592633No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC758030.15365143245304413No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG739430.14988124308899967No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA738260.1496440860161001No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG711670.14425433681233976No Hit
GCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA708250.14356110844540254No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA706940.1432955736030962No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGA693980.1406686029494394No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT685720.138994314554439No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA685700.13899026058738093No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGAT677160.13725921665356694No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG663880.13456738252698036No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC631140.1279310384528505No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA599340.1214852308304519No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG594450.12049403588474344No Hit
CGAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG584610.11849948409215219No Hit
CTAAACCATTCGTAGACGACCTGCTCTGTAGGCACCATCAATAGATCGGA578110.11718194479826567No Hit
TTCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA545800.11063276101588523No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG536080.10866253302564263No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG526770.10677541136009136No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG523300.10607204807550888No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC501220.10159646844335286No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA497330.10080797185055002No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG496690.10067824490469042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)