FastQCFastQC Report
Mon 3 Jul 2023
SRR5227299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48766213
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG42452528.705314066523886No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG11552002.3688532058046006No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG10572862.1680707501318586No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA9274341.901796229286863No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA6391611.3106635940748568No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5582321.1447105806637068No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT5528771.1337296172659541No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG4992581.0237784918833046No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA3544360.7268064879263846No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA3384430.6940112409384752No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG3265130.6695475820523525No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA3218540.6599938363062967No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3141810.6442595819363706No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA3020020.6192853236317529No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2983340.6117637225593056No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2966020.6082120832306581No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT2875560.5896623549587499No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2801650.5745063698097698No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2429180.49812766884318044No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2257200.4628614487657674Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1973260.40463671025675096No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1967250.40340429961211055No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1848890.3791333971329699No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1355870.2780347122709733No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1322640.2712205682241514No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1314640.26958008816472995No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1312440.26912895614838905No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA1311570.26895055394192696No Hit
GCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC1241490.2545799486213949No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1185150.2430268678029192No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1124180.23052435915005332No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG1095930.22473141394022128No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG1007750.20664922248524814No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG994890.20401215078972812No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC986760.20234501292934107No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGCTGTAGGCACCATCAATAGAT983290.201633454703567No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC948050.19440714004181542No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATAGATCGG933510.1914255675338169No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG909180.18643645755310134No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG880310.18051637513866414No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA851720.17465370952630666No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG837420.1717213514200908No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA790640.16212864427262377No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC790020.16200150706801858No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG770630.1580253935239958No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA740430.15183258129967975No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA675090.1384339604143549No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG674590.13833143041064108No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCG637160.13065603433262288No Hit
TCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG625480.12826093344586753No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT609320.12494716372583616No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG601090.12325951986470633No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA584970.11995395254497207No Hit
TCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC575350.11798127527351775No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATAG574280.11776186106557014No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG553360.11347200571018298No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA535540.10981783637782168No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA528430.10835985972501083No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT525630.10778569170421333No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA524590.10757242929648854No Hit
CCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATAGATC524180.10748835469344317No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG516250.10586222883454165No Hit
GCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA494750.10145343867484645No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC489180.10031125443347427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)