FastQCFastQC Report
Mon 3 Jul 2023
SRR5227301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49129512
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG43363468.826356752739576No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA12326352.5089502212030927No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG12057672.454262114388598No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG11551552.3512446042614874No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT8404621.7107069982702048No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT6802781.3846626443185512No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG6105121.2426583842314576No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA6050451.2315306531031693No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA5104401.0389681867794658No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA4471160.9100762083694216No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT3666990.7463925145440077No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG3146350.6404195506765872No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG3090100.6289702205875768No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3009880.6126419493033027No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2989650.6085242613441794Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT2276930.4634546339479212No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG2040290.4152880655521268No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA1951810.3972785237516709No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1863910.37938703726591055No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG1565460.31863943610919643No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1525360.31047733590351967No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1452180.29558201188727456No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1446090.29434243108297103No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1319730.268622655971018No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1297260.2640490302447946No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1202440.24474902172852844No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1188650.24194215484981815No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1179600.24010008485327514No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA1129350.2298720166404258No Hit
GCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC1087100.22127229759579128No Hit
TCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG1082090.22025254392919677No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGCTGTAGGCACCATCAATAGAT1068880.21756373236518203No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC1060890.21593741863342752No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATAGATCGG1057850.2153186459495059No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA991070.20172600126783266No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA973370.19812327873315738No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT899470.18308140329177297No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA897830.18274759171228894No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG895170.18220616561385752No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG881040.1793300938954981No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA821540.16721924695690035No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT755500.15377722457328702No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA751390.15294066018811667No Hit
CGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCGG726440.1478622462197467No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG711610.14484369394916846No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG668050.13597733272823878No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG661650.1346746533936669No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG660820.13450571216746465No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG648430.13198380639319193No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA612110.12459110116949665No Hit
TCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC605720.12329045727138507No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT583890.11884709947861888No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATAG576290.11730016776881479No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA570480.11611757918539879No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC566210.11524844781686412No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG561080.11420426891274636No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT556020.11317433806385051No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA554740.11291380219693613No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG549270.11180041845316926No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC543450.11061579443329297No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG539660.1098443640148512No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT537810.10946780826970152No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG520520.10594853862989724No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG517490.10533180138243586No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG510040.10381540121953584No Hit
ACGAGACTCTGGCATGCTAACTAGTTACCTGTAGGCACCATCAATAGATC500090.10179014194156866No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA491980.10013940297229086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)