FastQCFastQC Report
Mon 10 Jul 2023
SRR5227302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44258180
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT11289802.5508956762343145No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG10271412.320793579853487No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA6866511.5514668700791583No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4458471.007377619233326No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT4329680.9782779138229362No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4135710.9344509873654995No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3270570.7389752583590198No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2924490.6607795440300528No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA2703920.6109424291735449No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG2628790.5939670361501534No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2472770.5587147957733464No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2280200.5152041950211237No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2250800.5085613552116242No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2064580.46648551747948064No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG2050840.4633810066297349No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG2035300.4598697913018565No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA2017730.4558999037014175No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1999130.45169729076071363No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA1993910.45051784777412895No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1977740.44686428587890414No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1614610.36481617635429203No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA1580770.35717013216539856No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1278550.28888445028693005No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1168660.2640551418969329No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1117810.25256574038968616No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG1060100.23952634292688946No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1034040.23363816587125816No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG957500.21634418767332952No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG950840.21483938110423878No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG948600.214333259975896No Hit
TCAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGG757680.1711954716619617No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC756080.17083395657028824No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA725590.1639448346045861Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG721710.163068160507278No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG716790.1619565016003821No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG714990.16154979712224948No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG691950.1563439798021518No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG684070.1545635179756601No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC646320.14603402128148965No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA632280.14286172635205513No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT632240.14285268847476332No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG615000.1389573633619819No Hit
ACGAGACTCTGGCATGCTAACTAGTTACCTGTAGGCACCATCAATAGATC609500.13771465523435442No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT588970.13307596471431948No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGACTGTAGGCACCATCAATAGA573830.12965512815935948No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT565430.12775717392807384No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG560850.1267223369781586No Hit
GCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA548500.12393189236430417No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATCG543920.12289705541438894No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA538960.12177635863020123No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT525860.11881645381712488No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA520760.11766412446241577No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG515840.11655246555551992No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG508040.11479007948361185No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA500320.11304576916628746No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG499390.11283563851925227No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG499020.11275203815430278No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG482290.10897194597699227No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC481130.10870984753552902No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGA479030.10823535897770763No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA475680.10747843675451635No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA475650.10747165834654746No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG462210.10443493157649049No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA447670.10114966318090803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)