FastQCFastQC Report
Mon 3 Jul 2023
SRR5227305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48405034
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG32371026.6875317141601425No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG10490402.1672126085067926No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG10053192.076889358243194No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT8824361.823025266339034No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT8705651.7985009575656945No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA7757921.6027093380411632No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA6587611.3609348978042244No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA5010461.0351113481296181No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG4952881.0232158911405784No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA4893381.0109237811918488No Hit
TTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4549940.9399724830272819No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA4241000.8761485427321464No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATC4235950.8751052628121281No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA4109290.8489385628775717No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT3805530.7861847592132669No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2802980.5790678713292506No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG2493460.5151241087858754No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG2305750.47634508427367284No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCG2209010.45635955962762054No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1993770.41189311012569474No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1827090.37745867506259784No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1770170.3656995675284517No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1557020.32166489130035525No Hit
GCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA1401530.2895421992679522No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1365890.28217932870370466No Hit
TTTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1349400.27877265823219954No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1300340.2686373487517848No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1297580.26806716012223025No Hit
CCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG1290290.2665611184159069No Hit
GGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG1269110.26218554045432546No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1155480.23871070930349933No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA1136760.23484334294652082No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1128300.2330955908428863No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG1097620.22675740709117154No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1088870.2249497438634172No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA1038040.21444876993578807No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC1003030.2072160511239389No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG999120.20640828389873667No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGATC965710.19950610921996254No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC964480.19925200341766106No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG923710.19082932572674155No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA884800.18279090559052183No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG880710.18194595215034864No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG864190.1785330839763484No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT849670.17553339596869202No Hit
GCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC845610.174694640231014No Hit
TCGGGGAGGTAGTGACGAAAAATAACACTGTAGGCACCATCAATAGATCG845130.17459547699109146No Hit
TGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATAGATCGG801950.1656749171997276No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA785840.16234675095982784No Hit
CGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAAG771660.15941730358044992No Hit
GCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA762640.15755386103023913No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCG755570.1560932691422136No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA726100.15000505939113687No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG715430.1478007432036924No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC712200.1471334572350471No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGAT700460.14470808965860865No Hit
TGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCGG692470.14305743489406494No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA667080.1378121126823297Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG635430.13127353654993817No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGCTGTAGGCACCATCAATAGAT630880.1303335516715059No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCG617070.12748054262290157No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA613310.12670376391017513No Hit
AATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG607240.12544976210532152No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG604450.12487337577327184No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT573430.11846495139327864No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA571870.11814267086353042No Hit
ACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG565040.11673166059546618No Hit
TGCTAAGACTTCACCAGTCAAAGCGCTGTAGGCACCATCAATAGATCGGA543080.11219494236901063No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT540790.11172185107854692No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCGG540450.11165161045026846No Hit
TCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA523640.10817883115214835No Hit
TCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGATC521840.10780696900243888No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA510210.10540432633514936No Hit
AGTGCGGTAACGCGACCGATCCCGGAGCTGTAGGCACCATCAATAGATCG495340.10233233179838279No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA491170.10147085115155584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)