FastQCFastQC Report
Mon 3 Jul 2023
SRR5227306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57588015
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG17037742.95855656771639No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA12117802.1042225539463373No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT7510031.304095999836077No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA7131581.2383792009500587No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG5963511.035547066520699No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG5772451.002370024387887No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT4889880.8491141776635295No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA4680720.81279412044329No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4409570.7657096706667177No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA4066590.706152139468603No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3616820.6280508192546661No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3324750.5773336691671002No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3298480.5727719561092702No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3027930.5257916946781375No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2413530.41910282894800244No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2391530.41528258961521763No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2302410.3998071473725913No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA2293640.39828426105674936No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC2233600.3878584806230949No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2168660.37658182870168383No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG2039710.35419001679429307No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2030410.3525750974399795No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1983200.34437721112630815No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA1933350.3357208960927026No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1912780.33214897231654883No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1764090.30632936384419573No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1663640.28888649834518515No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1643160.28533020282084737No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA1623830.28197360162526874No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1567200.2721399582881959No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC1563830.2715547670813102No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC1462250.2539156801983885No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1426110.2476400688580775No Hit
CCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCGGA1417650.24617101318737933No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG1381200.23984157120192456No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1344200.23341662323315016No Hit
TTCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAG1340190.23272029779112893No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1333280.23152039534614974Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA1323100.22975266641852474No Hit
CCCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCGG1306320.22683886569106437No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1304150.22646205117505788No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1288950.22382261309058837No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1240640.2154337148102778No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1236310.2146818222506888No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC1149510.19960924161042884No Hit
CTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1139630.19789360685552368No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA1131970.19656346897874497No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1065530.18502634619373493No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1061920.18439947964867343No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1050140.1823539151332096No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1044500.18137454468607747No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG990180.17194202647894705No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG985620.1711501950536062No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG972690.16890493620938316No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC945080.16411053584673824No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG919200.15961654521344415No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT903370.1568677093662631No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC896390.1556556516143159No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG892980.15506351451773429No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG869440.1509758584316546No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC867920.15071191462320763No Hit
TGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT862680.14980200307303526No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA830930.1442887031268572No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG813340.14123424813305338No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAA801900.1392477236800053No Hit
CCCCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCG791560.13745221119359644No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC779730.13539796431601261No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT767990.13335934569024474No Hit
CGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGAT737280.12802663887616197No Hit
GGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC729250.12663225151969554No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG727880.1263943547976085No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG713200.12384521327918666No Hit
GGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT701450.12180485818099479No Hit
TCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAA700310.12160690032465957No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT693360.12040005198998437No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCA691130.12001281863943392No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA687700.11941720859800428No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG659980.11460370703869546No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA655700.11386049684122643No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG642470.11156314382428359No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATA641980.11147805667550792No Hit
TGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCGG635310.11031982956870452No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC627600.10898100932980587No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA626820.10884556448073442No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA618700.10743554887245202No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG610990.10609672863355335No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAG609670.10586751427358626No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAGA603700.1048308402364624No Hit
TGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA590310.10250570366073565No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA582220.10110089746972525No Hit
TGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT581050.10089773019611806No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG577660.1003090660443844No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT577090.10021008711621682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)