FastQCFastQC Report
Mon 3 Jul 2023
SRR5227309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5227309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40062613
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG13453333.3580760196545345No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG9579912.391234440948722No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA6377201.5918083026686254No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT4533041.1314888522124107No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG3709180.9258457505005976No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA3696580.922700673568147No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA3325310.830028236051403No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA3184200.7948058705007585No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2504600.6251714035727026No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2486270.6205960654638278No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2222980.5548764380396256No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2199050.5489032879607728No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2094200.5227317549157365No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC2027830.5061651869786926No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1922530.4798813297574973No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1914700.47792688909233155No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1895190.47305701203264No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG1675700.4182702710879093No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC1593160.39766752108755365No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1491020.3721724291922746No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1439380.35928260595483374No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1397620.34885892240728283No Hit
CCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCGGA1328740.33166583517655224No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA1322370.3300758240607022No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1297260.3238081350310325No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1293850.322956967385028No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1291050.322258061400039No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1280410.3196022186570806No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT1186600.296186372067144No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1135640.28346628314034333No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1021710.2550282978296997No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1000520.24973907717901478No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATA1000430.24971661234378298No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA907940.22663025000391265No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG874690.2183307414321677No Hit
CTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG857150.213952594654772No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA828540.2068112731438661No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC813160.20297228241203338No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG806270.20125247447039962No Hit
TTCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAG741480.18508028919631378No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA726800.18141602496072834No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA715000.17847063545256023No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC703910.17570246853344287No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG701950.17521323434395056No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC692570.17287189929423724No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC678340.16931995923481077No Hit
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGAT670410.16734055764160966No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA668050.16675147973997603No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA641540.16013433771781188No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATCA634840.15846195553944523No Hit
GGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC631780.15769815114156432No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG626070.1562728821507474No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA622950.15549410119604531No Hit
GGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG609170.1520544853127778No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG604220.15081891937502928No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA603070.15053186870262306No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC601780.1502098727309674No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG581340.14510785904054735No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA579320.1446036482942338No Hit
CCCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCGG570020.14228228198694878No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA560310.13985857587471892No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC557340.1391172363120698No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG553510.13816123276831693No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT552190.13783174851825067No Hit
GGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT544720.13596716719401203No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA529450.1321556334830182No Hit
CCCCCCCCGAGTGTTACAGCCCCCCGGCTGTAGGCACCATCAATAGATCG499660.12471977302129543No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG492910.12303491037891114No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA484440.12092071977431926No Hit
AATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG481140.1200970091491536No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAG472430.11792291231727697No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC467130.11659998313140485No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAA460360.11491012830341346No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC457530.11420373404001383No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA456280.11389172243957228No Hit
TGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA454060.11333758983718811No Hit
TCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAA438370.10942122022844591No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT430630.10748924439851189No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG427140.10661810801007912No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA425410.10618628395506803No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT421660.10525024915374341No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT419160.10462622595286034No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT416850.10404962851524437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)