FastQCFastQC Report
Wed 27 Sept 2023
SRR522871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30099263
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATG22652947.526077964101646TruSeq Adapter, Index 8 (97% over 37bp)
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACG6069442.0164746226510597No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG5552651.8447793887843698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCAC5148131.7103840715302563No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT4181031.389080523333744Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG3863501.2835862459489458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG3789541.2590142157301327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA3743831.2438277973782945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA3208561.0659928782973855No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA2456170.8160233026303667Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT2140160.7110340209991188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2012220.6685279968482949No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG1506160.5003976343208137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA1471800.48898207241818514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT1457350.48418129041897134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1435140.4768023722042629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACG1400470.46528381774663385No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACAC1398430.46460606028792134No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTAGATCGGAAGAGCA1313170.43627978532231837No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCT1284790.42685098302905294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT1257810.4178873084035313No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC1253490.41645205731449303No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1243920.41327257747141516No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCACA1183680.3932587983964923No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC1174510.3902122121727698No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA1134380.3768796598109395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA1108000.3681153256144511Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA1078590.3583443222513455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGT945760.31421367360390184No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA924000.30698426071096824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA895660.2975687477796383No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGT830160.27580741761019195No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTTAGATCGGAAGAGC785280.2608967535185164No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACAC783210.2602090290383522No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCAGATCGGAAGAGCACACGT779280.2589033492281854No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCAC763260.2535809597730017No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG735420.24433156386586607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG718400.23867694036229392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTC717470.23836796269729263No Hit
CCCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCT716330.23798921588212973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT715860.23783306587938716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGCACACGTCTGAACTC711130.23626159883050954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGGACGCTTTCTGGTGTGTGCTTGGACCAGATCGGAAGAGCACACGTC662880.22023130599576474No Hit
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA614250.20407476422263227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC584360.19414428851630022Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT576550.19154954059838608No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC568350.18882522140160043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACAC498080.16547913482134097No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTC493810.1640604954347221No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCT483080.16049562409551357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTC478170.15886435491792608No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC473820.1574191368074361No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA444000.14751191748449125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT442400.14698034300706964Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTC418940.13918613223187556No Hit
TTGCGGACGCTTTCTGGTGTGTGCTTGGACCTAGATCGGAAGAGCACACG418100.13890705563122926No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC417250.13862465669009902Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG413290.1373090098584806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA397790.13215938210845893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC397780.13215605976797506No Hit
CGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACG392160.13028890441603172No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGA379170.12597318412746517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC362240.12034846168824799No Hit
GGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACG357120.1186474233604989No Hit
TGCGGACGCTTTCTGGTGTGTGCTTGGACCTAGATCGGAAGAGCACACGT352420.11708592333307298No Hit
TTGGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACAC327420.10878007212336063No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATCGATCTCGTATGC326400.10844119339400435TruSeq Adapter, Index 8 (97% over 37bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACA321770.10690294974996564No Hit
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGT318120.10569029547334763Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACG316230.10506237312189338No Hit
CCGCCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACG313350.10410553906253452No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG307880.10228821881784947No Hit
GCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCA306070.1016868751902663No Hit
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC303000.10066691666171361Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)