FastQCFastQC Report
Wed 27 Sept 2023
SRR522872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59233837
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG15519562.62004975298156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC12405852.094385680265825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG9745161.6452015424899793TruSeq Adapter, Index 10 (97% over 36bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA9272151.5653468472758232Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGA7837641.3231693904955033Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC6846541.155849485151536No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA6638151.120668580021247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAAC6412591.082588993854982Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6053871.0220290135855963No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACT5904640.9968356431139181Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT5787900.9771273132280794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGCACACGTCTGAACTC5423390.9155898511183735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA4661490.786964045567401Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGA4380830.7395823437877239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGA4292340.7246432474060392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAA4188930.7071853204444615Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC3774370.6371982959672189Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA3668000.6192406546278607No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTGAA3447280.5819781689982366Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA3352000.5658927683513058Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG3248700.5484534118564698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTAGATCGGAAGAGCAC2837350.47900830736323896No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAGATCGGAAGAGCACA2779690.469274006342017No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG2675310.45165232162826124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACT2671800.45105975491677164Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAACT2548900.43031147889338994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTGAA2370630.40021550520186633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAGAGCACACGTCTG2249850.3798251327193273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTC1748540.29519276287977086No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG1620470.2735716749195228No Hit
TGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAA1562640.26380867408606334Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCT1507500.2544998055756543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTG1492460.2519607163047702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCACA1306480.2205631217170686No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG1234300.2083775190859238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGATCGGAAGAGCACAC1229420.2075536656522859No Hit
TTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAACTC1129070.19061233531098112Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACT1051440.17750665046399072Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCCTAACTGAGATGCAAAGATTGTGTAGATCGGAAGAGCACACGTCT971230.1639654037606917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC970940.16391644525746324Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATATTCCCTAACTGAGATGCAAAGATTGTGTAGATCGGAAGAGCACACGT900640.15204822878517898No Hit
TATTCCCTAACTGAGATGCAAAGATTGTGTAGATCGGAAGAGCACACGTC899550.15186421234200986No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC888900.15006625351655No Hit
TTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAACTC879490.14847763449799817Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACTC867510.14645514184738698Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTGAAC852440.14391098790375506Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGGGACGTTGTTTCCGAAAAGCACCGCGAGATCGGAAGAGCACACGTC839590.14174161974345847No Hit
GCGGAAACCATTTTTATCGCATTGGTTTGAGATCGGAAGAGCACACGTCT819260.1383094598447168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGATCGGAAGAGCACACG810580.1368440811963608No Hit
ATCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGT802860.13554077207593357No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC747380.1261745039410498No Hit
GTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGT730610.12334335187504399No Hit
TTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAACT723650.12216834779756036Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC714350.12059829924575037No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTAGATCGGAAGAGCACACGTCT711670.12014585514694918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAAC701820.11848295426142999Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAAGAGCACACGTCTGA684400.11554206761922245Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGTCAAGGTGTTGTATTCAGTAGATCGGAAGAGCACACGTCTGAACT682300.11518754052687824Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTGAACT673230.11365632113280116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGAGCACACGTCTGAAC668200.11280714433542435Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGAAACCATTTTTATCGCATTGGTTTAGATCGGAAGAGCACACGTCTG649750.10969237059554322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATACTGCGATCTGTTGAGACTATAGATCGGAAGAGCACACGTCTGAACT647400.10929563789696757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT647380.10929226144846907Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGACGTTGTTTCCGAAAAGCACCGCGAGATCGGAAGAGCACACGTCT647260.10927200275747796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAAC642220.10842113773585188Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTAGATCGGAAGAGCACACGTC636790.10750443196850476No Hit
CCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC635950.10736262113156707Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTTGAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACG625380.10557816810010129No Hit
ATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGAGCACACGTCTGAACTCC620010.1046715916782497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCTG614900.10380890908687884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGAGATCGGAAGAGCAC609980.10297830275624387No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTAGATCGGAAGAGCAC606980.10247183548146643No Hit
ACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAA606050.10231483062628545Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATATTCCCTAACTGAGATGCAAAGATTGTGTAGATCGGAAGAGCACACG596440.10069244712274843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)