FastQCFastQC Report
Wed 27 Sept 2023
SRR522896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR522896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20213950
Total Bases727.7 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTA9324124.61271547619342No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG8590834.249951147598565No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA6501993.2165855758028488No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG5304382.624118492427259No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT4997332.472218443203827No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG4708182.3291736647216403No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGA4449052.2009800162758886No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA4105052.03080051152793No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCAG3583201.7726372134095514No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGT3223001.594443441286834No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAA3030721.4993210134585273No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC3023391.4956948048253804TruSeq Adapter, Index 6 (100% over 35bp)
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGA2686461.3290128846662825No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGT2596911.2847117955669227No Hit
GTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAGA2338161.1567061361089743No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAG1751850.866653969164859No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCA1701150.8415722805290407No Hit
TGTTTGTTGACTCTGAATAAAGCAGTTTACTGTCAG1608760.7958662210997851No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT1486080.7351754605111815No Hit
TGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGA1466000.7252417266293821No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAA1390440.687861600528348No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGA1346110.6659312009775428No Hit
TATACTGCGATCTGTTGAGACTATCCTTTGATTGAG1288110.6372381449444566No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGG1188530.5879751359828237No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGA1139080.5635118321753046No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG1094470.5414429144229604No Hit
TTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAAG1027650.5083865350413946No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTT1021470.505329240450283No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAGA1014220.5017426084461474No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGA1012030.500659198226967No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGAT972220.48096487821529194No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTT913310.451821638027204No Hit
AGAGAAGGTGTCTTGCTGGCGGTAGATCGGAAGAGC913120.45172764353330247No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG835820.4134867257512757No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAG791610.3916156911439872No Hit
TGGTCGTGACTTCTAATTTGCTGGTTTGAGGTTGGG774680.3832402870295019No Hit
TGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAA696130.34438098441917586No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGA671840.3323645304356645No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAG667090.3300146680881273No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAA644500.3188392174711029No Hit
TTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAG625250.3093160911152941No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA618100.30577892989742234No Hit
GCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCT539360.2668256327931948No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCA510200.25239995151862943No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGA505930.25028754894515914No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCAG494850.24480618582711444No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATAGATCGGAA464790.22993526747617363No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGAT452650.22392951402373115No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAC434080.21474278901451718No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGAA422510.20901901904377918No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAA420340.20794550298185166No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTAGTAGATCGGA413660.20464085445942035No Hit
TTTTTTTCGAAGCATTAAGCGGAGCAGATCGGAAGA400530.19814534022296482No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAA397880.19683436438697036No Hit
GTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAG396060.19593399607696665No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGA363920.18003408537173587No Hit
TGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAG360110.1781492484150797No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA350820.1735534123711595No Hit
TTACTGCGATCTGTTGAGACTATCCTTTGATTGAGT343520.1699420449738918No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGTA339910.1681561495897635No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGA335280.165865652185743No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA303950.15036645484925015No Hit
CAATGAGTTGAATACAAATGATTCTTCGAGATCGGA299640.1482342639612743No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCAAGATC289990.14346033308680392No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGA282480.13974507703838193No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGG270070.13360575246302678No Hit
TTACCACAACGCAAGGCGGGGTGCAATTATTAGAAG256060.12667489530744858No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAA254210.12575968576156565No Hit
TCCCATGTGGTCTAGTGGTTAGGATTCGTGGTTAGA253010.12516603632639836No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAA245490.12144583319934996No Hit
TGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAG232980.11525703783773088No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGAG229770.11366902559865835No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAG228460.11302095829860072No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTAGA227150.11237289099854308No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATAGATCGG220340.10900393045396868No Hit
TTTTTCGAAGCATTAAGCGGAGCCATTAGATCGGAA218850.10826681573863593No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTAGATCGG211180.10447240643219162No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATTAGATCGG203720.10078188577690159No Hit
CTAATTTGCTGGTTTGAGGTTGGGTAGATCGGAAGA202390.10012392431959116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)