FastQCFastQC Report
Mon 3 Jul 2023
SRR5262885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56957808
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG56678599.950978099438096TruSeq Adapter, Index 2 (100% over 49bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC9751261.712014619663734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC8458791.4850975304386715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT5656010.9930174981452938Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC5372710.9432789267452146No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT3785840.6646744551686399Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT3063450.5378454873122926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC2972840.5219372206177597Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2841490.49887629102580633No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2615440.45918901935271106No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2550650.4478139327271864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2394290.42036203359511304Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2317220.4068309651242196Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2156990.37869961568745764Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2034480.35719071211448306Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA1975540.34684270153092966No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1897090.3330693484552636No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA1823740.3201913950059314Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1809170.3176333611714833No Hit
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1615090.2835590161756225Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG1508210.26479424910453014No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1337320.234791338880176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1215000.2133157933324962No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1197080.21016960484153466No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG1185480.2081330096130104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1167820.2050324689461364No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1161250.20387898354515327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC1148240.20159483665523084No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC1136990.19961969042067068No Hit
GTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCA1080100.18963159537319274Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1052040.18470514174281424No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG964170.16927793288674312No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC911740.1600728735909219No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT887090.1557451087303079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTC804130.14117994147527588No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACGT803710.14110620268251897No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC792850.13919952818409023Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA780840.1370909498483509No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC771120.13538442350169094TruSeq Adapter, Index 2 (100% over 50bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC759650.1333706521852105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA757740.13303531624672071Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC752730.13215571779026328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA735340.12910258063301874Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG734990.12904113163905465No Hit
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG729590.1280930614464658Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT727970.1278086403886891No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC716030.1257123518517426Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA705600.12388117183161262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC630530.11070124046908547Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGAATTGAGCAATGAAGTACGCACAAGATCGGAAGAGCACACGTCTGAA607220.10660873747107684Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT600280.10539029170504595No Hit
GTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTCCAG599940.10533059839662369Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG579090.10166999404190553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)