FastQCFastQC Report
Mon 3 Jul 2023
SRR5262886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72029787
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG48143836.683877879577792TruSeq Adapter, Index 3 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC7909561.098095708654532Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC5714030.7932870882986229Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC4006430.556218498882969No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT3115060.43246830647992895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2750530.38186007685959145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2595090.360280115780434Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2394100.3323763820098482No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC2163130.30031048127353205No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACGT2054900.2852847530980482No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT1784910.24780164905943702No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1633090.22672425784071804Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1483030.20589120997956029Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGTC1455400.20205529692875532No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1352240.1877334442207916No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC1351810.1876737466959329Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTAAAGCATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTC1334510.18527196255626857No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1333200.18509009335262924No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGT1278650.1775168375827628No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC1264790.17559263364196814Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACG1248180.17328664320498408No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1215360.16873019491227984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1142750.15864964309834764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1109560.15404182716797427Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC1068470.1483372427576386Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1036610.14391407265996772No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGT1007470.13986852411489153No Hit
AGGCTTCAAGGCTCACCGCAGCGGCCCTCCTAGATCGGAAGAGCACACGT960810.13339064851045582No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCAC958620.13308660762803587No Hit
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG910590.12641853293277128Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG864480.12001701462757346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC859830.11937144837038044No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA844780.11728203500032564No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG831430.11542863510064247No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG793820.11020718414730285No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA786110.10913679364344088Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC744840.10340721957153642No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG742900.10313788655240644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT736060.10218827941279349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)