FastQCFastQC Report
Mon 3 Jul 2023
SRR5262887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82714832
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG19350392.339409937990323TruSeq Adapter, Index 4 (100% over 49bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT4851750.5865634835599981Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4501130.5441744716352684Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3787480.4578961122716178Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC3720030.44974158927143804No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC3643690.4405122892590775No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC2231390.26976903005739045No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT2029360.24534414819339778No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1859520.22481095047137373No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1659230.20059642991235235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1524690.1843309069406077Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1392430.16834102981675644Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1383020.16720338620768763No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACGT1330290.16082847148864426No Hit
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG1286220.15550052740238895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGT1266360.15309950699047542No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1146840.1386498614903794No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT999720.12086345046315272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC998610.12072925445825726Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGTC985040.11908867807408471No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA969970.1172667557373507No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA905740.10950152204866957Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCAC887060.10724316045277105No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACG855600.10343973134104897No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA854340.10328740074089734Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGCTTCAAGGCTCACCGCAGCGGCCCTCCTAGATCGGAAGAGCACACGT837570.1012599529912604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)