FastQCFastQC Report
Mon 3 Jul 2023
SRR5262888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101519573
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG36697203.6147906177658964TruSeq Adapter, Index 6 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC14928401.4704947586806734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC9182910.9045457667557368No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA8108910.798753359610762Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4896210.48229221767904795Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT4404990.4339054893384943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3926760.38679831720726404No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACGT3750980.3694834295648584No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC3631160.35768077944929894No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC3248540.3199914956301087No Hit
ATAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGTC3219690.3171496791067078No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2788430.2746692009825534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT2684650.26444654175210136No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2382500.23468380821499318Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT2282990.22488175753063894No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT2194550.2161701369646226No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT2136890.2104904440447164No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2083560.20523726986125126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1955630.19263575901762314No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACG1937210.19082133058223166No Hit
TATAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGT1910040.18814499938844306No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1771990.17454663644024587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1710760.16851528719491363Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1644890.16202688322969996Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1526150.15033061654031976No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1392580.1371735478044219No Hit
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG1280990.12618157879761768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA1252370.1233624180038661Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1220250.12019849610675568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1173800.11562302374932172No Hit
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC1167530.11500540885844744Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1127730.11108498259739527Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCAC1114600.10979163594393762No Hit
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1034740.10192517259701239Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGTCT1021580.1006288708483831Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1018280.1003038103795019Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)