FastQCFastQC Report
Tue 4 Jul 2023
SRR5262889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105009484
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG54032285.145466670419978TruSeq Adapter, Index 12 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC15018301.4301851059471924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC11955651.1385304969215924No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC7016400.6681682199295447No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC6111220.581968386779236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5844740.556591631285418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA5159550.491341334464609No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT3718980.3541565826568579No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT3028690.2884206154179369No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG2218870.2113018667913843No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTC2108140.20075710494873014No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2083220.19838398596454393Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC2061840.19634797938822365Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1998250.19029233588082387No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1923440.1831682174535778Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1856290.17677355694843713No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1805360.17192351883188Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1745490.16622212904122072No Hit
CGCCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACT1732480.1649831933275665Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1619890.1542613046265421Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAACCTAGACTTACAAGTCAAAGTAAAATCAGATCGGAAGAGCACACGT1606650.153000466129326No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT1527470.14546019481440361No Hit
CGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAAC1421790.13539634191517405Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1419260.13515541129599304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT1386280.13201474259220242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC1377550.13118338911178729No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1359090.1294254526572095No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1348030.12837221445636282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAACCTAGACTTACAAGTCAAAGTAAAATAGATCGGAAGAGCACACGTC1265920.12055292072476044No Hit
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1189910.11331452690501746Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG1167750.11120424132357416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)