FastQCFastQC Report
Mon 3 Jul 2023
SRR5262890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99685391
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG67848276.806240043739208TruSeq Adapter, Index 6 (100% over 49bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT8544510.8571476636932687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG7538630.7562422060420066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT5779940.5798181601153574No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATAGATCGGAAGA5287700.5304388082301849No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT5281870.5298539682710378No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGT5050180.506611846464042No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC4366430.43802105365669874No Hit
ATAGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACG4357240.43709915327512733No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT4250400.42638143436684717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG4087760.41006610487187634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC3760180.3772047199975371No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA3666950.3678522964312795No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT3662670.36742294565509603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG3635820.3647294717437583No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACGTC3621470.3632899428563209No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3247970.32582206554218157No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC3147380.31573131914585156No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA3144530.31544541968040235No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC3100630.31104156475646466No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2981480.29908896078864755No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT2946970.2956270693666638No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCAGATCGGAAGAGCACAC2918660.2927871346765345No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT2880850.2889942017682411No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACGTC2793150.28019652348055696No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA2711590.27201478298861265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT2708780.2717328961472399Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA2518370.25263180238717226No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC2426270.24339273545107526No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC2386600.23941321552322548No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGGCTGAGATCGGAAGAGCACACG2373350.23808403379789123No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC2329390.23367415993783883No Hit
AAATACCGGCACGAGACCGATAGTCAACAAGTAGATCGGAAGAGCACACG2278100.22852897271577136No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA2277930.2285119190634463No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2269150.22763114807865878No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACG2257610.22647350603259409No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2246610.2253700344115619No Hit
ATCGCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTC2084110.2090687491008587No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAG1980350.19866000224646757No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC1898030.19040202189707015No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1881910.1887849343942484No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG1710810.1716209349071019Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGAGGCCCCGGGTTCGATCCCCGGCATCTCCACCAAGATCGGAAGAGCA1681320.16866262780671643No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCA1579320.15843043641169044No Hit
ACCGGCACGAGACCGATAGTCAACAAGTACCGTAGATCGGAAGAGCACAC1574440.1579408962743598No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1514040.15188183391887383No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT1486660.14913519273852274No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC1478150.14828150696625148No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA1437750.14422875664900586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG1419450.1423929811340159Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGGATTAGCACTACGCATCGGTTACAAGATCGGAAGAGCACACGTCT1410820.1415272574895152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC1367120.13714346568595995No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGAGATCGGAAGAGCACA1351900.13561666222485902No Hit
GGGAGAGTTCGTCCAAGTGCACTTTCCAGTAGATCGGAAGAGCACACGTC1339520.13437475507318822No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC1308650.13127801244216417No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1284460.12885137803191243No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGC1271580.1275593130792856No Hit
ATTGTTAGGACCTAAACTCAATAACGAAAGTAGATCGGAAGAGCACACGT1254140.12580980898194No Hit
AGGTAAGGGAAGTCGGCAAGCCGGATCCGTAGATCGGAAGAGCACACGTC1186710.11904552794501254No Hit
CTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAAC1139060.1142654895139048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC1114120.11176361840221904No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA1105400.11088886635354622No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGATCGGAAGAGCACACGTC1104520.11080058862386365No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATTAGATCGGAAGAGCACACGTC1093800.1097252053713668No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC1083850.1087270651323422Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCATATTAGTCAGCGGAGGAAAAGAAACTAGATCGGAAGAGCACACGT1021390.10246135263691748No Hit
CGGCCGCAGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCA1011590.10147825973817969No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCT1010070.10132578002327343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)