FastQCFastQC Report
Mon 3 Jul 2023
SRR5262891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105725466
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG69867176.608357725280681TruSeq Adapter, Index 12 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC19501481.8445395170923153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC12919511.2219865741712597Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA6598520.6241183179083837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC6266430.5927077209572195No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT5201210.491954322528122Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA4040810.38219836269153923No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3740320.35377663882796223No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT3686680.34870312134637454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3679200.3479956286028571Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA3036310.28718814065099507Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT3016990.2853607663455463No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2729040.2581251332578662No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT2706170.25596198365302075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC2675260.25303837393348544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2670170.2525569383633646Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC2269800.2146881055128194No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2233880.2112906269904736No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2198340.20792909061285195No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2152450.2035886037144542Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA2062290.19506085695569314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA1901760.17987719250156817Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1857990.1757372249368946No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1855610.17551211360941177No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCAGATCGGAAGAG1703490.161123905568787No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT1679230.15882928338192426No Hit
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC1632770.15443488326644028Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC1486110.1405631070947467Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAGATCGG1472210.13924838127457392No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1397770.13220750429229605No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1396420.13207981509393393TruSeq Adapter, Index 12 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA1363100.12892825650917444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC1320220.12487246923082845No Hit
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1292040.12220707544575873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA1270010.12012337689767194No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG1256910.11888431875060262No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA1238220.11711653273772281Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG1237170.1170172189167745No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1216440.11505648033748085No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1117480.10569638917458164Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT1112090.10518657822704701No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG1111120.10509483117340906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC1098890.10393806162083978No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1096870.10374700074625351No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACG1087070.10282007175073601No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1080370.10218635498849445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1077250.10189125106339092No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1075310.10170775695611502No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1065500.1007798821146837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)