FastQCFastQC Report
Mon 3 Jul 2023
SRR5262892_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262892_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84490800
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG994744211.77340254796972TruSeq Adapter, Index 6 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC26248353.1066518484852788Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA10417791.233008800958211Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC6900610.8167291586776312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA6753390.7993047763780199Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5546650.6564797587429638Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5487030.6494233691715547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4739440.5609415462985319Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT3545310.41960899884957886Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3285770.3888908614902451No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2964420.35085713474129726No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG2640640.31253580271461506No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2596650.3073293186950532No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC2080120.24619485198388463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2077270.24585753715197395Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG2039450.2413813101544784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG2037700.24117418701207705Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC2033090.24062856547695136No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1820110.2154210872663059Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTTAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC1799070.21293087531423538Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCAGATCGGAAGAG1710440.20244097582221968No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1597850.18911526462052675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1569810.185796560098851Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1485150.17577653424988282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTC1398710.1655458345760722No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAGATCGG1376770.1629491021507667No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC1319010.15611285489070997No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC1176770.1392778858763321No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCAGATCGGAAGA1156810.13691549849214352No Hit
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAACT1147960.1358680471719998Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1068810.1265001633313923TruSeq Adapter, Index 6 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1001760.11856438807538809No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC998130.1181347555000071No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC934780.11063689774507993TruSeq Adapter, Index 6 (100% over 50bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC907200.10737263702083541No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC875150.10357932461285725No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC869170.10287155524625166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA863580.1022099447513812No Hit
GGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAAC847720.10033281730081854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)