FastQCFastQC Report
Fri 7 Jul 2023
SRR5262893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190266869
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC61690643.2423217097244605Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA47135362.477328830170638Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC35604181.8712758656894701Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG30302761.5926451178423502TruSeq Adapter, Index 7 (100% over 49bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT20723421.0891764871581504No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG11554050.6072549603998582Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG10898740.5728133361988523No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA9516890.5001863987155851No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT8394270.4411840087619248No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC8348280.43876687748511806No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC7923850.4164597883828109No Hit
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT7870280.4136442693026078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC7601570.399521474229967Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT7585530.3986784477963949No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC7474310.39283297398455636No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT7225310.37974609231626133Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT6841460.3595717970215824Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT6435070.3382128498682553No Hit
GCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGA6103590.3207910043445346Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC5805870.30514350872090085No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACA5642140.29653822705202554No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAAC5070990.2665198637393881Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA5050810.2654592481889214No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT4963440.2608672768983233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACG4747360.24951059661364375No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG4511360.23710696579550064No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC4494130.2362013956302608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4378470.23012256537421655No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC4171320.21923522586583372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3949950.20760051504290009No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3798520.19964169379378394No Hit
TCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT3767370.19800451964130444Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA3602140.18932040133587313Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCAGATCGGAAGAGCACACGTCTGAA3560330.18712296148626906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG3418210.1796534529613771Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC3393620.17836105769943586No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATAGATCGGAAGA3301610.17352521841309113No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC3265030.17160265563627897No Hit
CCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCA3026890.1590865512166493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2998520.1575954876305869No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC2976410.15643343560775155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG2893530.15207744864924436No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACA2801360.147233200121667No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2774340.14581308950850502No Hit
TCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAG2709060.14238211908558815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACA2654430.13951088878221884No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2613740.13737231362124322No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC2597890.1365392731616349No Hit
GGGCCAGCTTTAGCTCAGCGGTTACTTCGACAGATCGGAAGAGCACACGT2543450.13367802883222932No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT2539250.13345728624987255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGGAAGAGCACAC2505800.13169922925467387No Hit
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2439680.12822411031528563Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGCCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTG2350330.12352807466443357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAAC2273990.11951581544131049Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2233900.11740877493495727No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC2184680.11482188210076658No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2176120.11437198769482038No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2156660.1133492137299006No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC2088200.10975110963748502Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG2079470.10929228041272913No Hit
CTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA2025530.10645731496217559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACG1941790.10205612833204293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)