FastQCFastQC Report
Mon 3 Jul 2023
SRR5262894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72862561
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG862142411.832447119172768TruSeq Adapter, Index 8 (100% over 49bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT9289501.2749345991283507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC8843431.2137138577931676No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA7627421.046822935581416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC6746960.9259844709548434No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT5683900.780085124924445No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT5026430.689850854954165No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT4579990.6285793330816357No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG4197700.5761120584273727No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA3536660.48538782489404947No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3152970.432728407666044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT3085680.42349321210381285No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC2865790.3933144760036639No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC2558620.35115702287763395No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2134570.2929584097380272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCTG1956760.26855493042579165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1869900.25663385617203327No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1857490.2549306495005027No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1767560.24258823403146643TruSeq Adapter, Index 8 (100% over 50bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC1735540.2381936588805875No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1496900.20544158473924626TruSeq Adapter, Index 8 (100% over 50bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT1492920.20489535085103586No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1474700.2023947524984745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1348940.185134859588589Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACG1300970.1785512315440024No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1247700.17124020661310546Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCTGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1239810.17015734596537171TruSeq Adapter, Index 8 (97% over 49bp)
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG1162010.15947970865311747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1090200.1496241670670895No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC987580.13554011641177421No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT959880.13173843834558602No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAGATCGGAAGAGCACAC955770.13117436264695664No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC872280.11971580301713525No Hit
TTGAATTGAGCAATGAAGTACGCACACAGATCGGAAGAGCACACGTCTGA832590.11426856105153922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA828810.11374977610243482No Hit
GCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGA789150.10830665147770471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTG771580.10589526217723805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCTTATG771480.10588153770768503TruSeq Adapter, Index 8 (97% over 49bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC765320.10503611038321863No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC755060.10362797980707815Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)