FastQCFastQC Report
Mon 3 Jul 2023
SRR5262895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5262895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53428814
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG22816134.27037927512297TruSeq Adapter, Index 7 (100% over 49bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC7667081.4350084581701552No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA7282741.3630734906449542Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC4655600.8713650278667986No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3294270.6165717996285675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACAC3033100.567689935995959No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2841220.5317767300617978No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGTC2804200.52484788451415No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA2691450.5037450391468544No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA2474690.4631751698624641No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG2303130.4310651552175573No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2254600.4219820413756517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCAGATCGGAAGAGCA2148410.4021069979206351No Hit
TTACTAAAGATTTCCGTGGAGAGGAACAAGATCGGAAGAGCACACGTCTG2098810.39282361760828155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC1892060.3541272692296707No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1797560.33644018375552937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1536450.2875695500184601No Hit
GCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGA1513360.2832479118851487Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA1335690.24999431954450643No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAGATCGGAA1295680.24250585086915835No Hit
TTACTAAAGATTTCCGTGGAGAGGAACAGATCGGAAGAGCACACGTCTGA1258380.23552459914232796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG1258160.23548342285868445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGA1208000.22609523018796562No Hit
TTTTTGAGGCCTTGTTTCGGCAAGGCTAGATCGGAAGAGCACACGTCTGA1101510.2061640372552533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAA1039760.19460660309622443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1011610.1893379104391125Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT999440.18706011329392414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG968250.18122243926282924No Hit
GCCGCCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTG952130.17820534066131433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGA949930.17779357782487928No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAG948940.1776082845484835No Hit
GGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCT896740.167838275429434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTAAGATCGGAAGAGCA891340.16682758483091165No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC888470.16629042149428958No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA878890.16449738150654064No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC855270.16007654596263357Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGAGATCGGAAGAG824150.15425197347633432No Hit
TTTTTTGAGGCCTTGTTTCGGCAAGGCTAGATCGGAAGAGCACACGTCTG814800.1525019814214854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAAGATCGGAAGAGCACACG795750.1489364895878093No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC781960.14635548526306422No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG759290.14211245639852685No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG751000.14056085916486935No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAGATCGGAAG735650.13768787755610673No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT721780.13509190003730945No Hit
TACTAAAGATTTCCGTGGAGAGGAACAAGATCGGAAGAGCACACGTCTGA716940.13418602179715236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT712280.1333138332436127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC712260.13331008994509966No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTAGATCGGAAGAGCAC708070.13252586890661655No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGAGATCGGAAGAGCACACGT693440.12978764604432358No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT689960.1291363121030536No Hit
TTTACTAAAGATTTCCGTGGAGAGGAACAAGATCGGAAGAGCACACGTCT686050.12840449724375316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTAAAGATTTCCGTGGAGAGGAACAAGATCGGAAGAGCACACGTCTGAA677680.1268379268160435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCA676680.1266507618903912Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC663130.1241146771478027Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGGCCGCCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTG647820.12124918213606614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG644340.12059784819479617Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG643630.12046496109758303No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT622800.11656631569624586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAG609160.11401338611034863No Hit
GGGCCGCCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTC602090.11269013008598694No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAGATCGGAA585530.10959067891718503No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC578500.10827490948984943Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA572240.10710325705526609Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAACTCC544260.10186638243551505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)