FastQCFastQC Report
Mon 3 Jul 2023
SRR5266618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5266618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8880659
Total Bases310.8 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGNNNNNNNNNNNNNN1466501.6513414150909296No Hit
AAGGCTGAGGCGTGATGACGAGNNNNNNNNNNNNN1454531.637862685640784No Hit
AGCGAAATTCCTTGTCGGGTNNNNNNNNNNNNNNN959471.080404055599928No Hit
ACGAGAGGACCGGAGTGGACGNNNNNNNNNNNNNN930091.0473209251700804No Hit
GAGAGAACTCGGGTGAAGGAACNNNNNNNNNNNNN894081.0067721325635857No Hit
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGANNNN602440.678373080195963No Hit
AGGCTGAGGCGTGATGACGAGGCACNNNNNNNNNN586360.6602663158218326No Hit
GAGAGGACCGGAGTGGACGCNNNNNNNNNNNNNNN529460.5961944941248166No Hit
ATGACTTGTGGCTGGGGGTGNNNNNNNNNNNNNNN513940.5787183135846112No Hit
AGGCTGAGGCGTGATGACGAGGNNNNNNNNNNNNN489540.5512428751064532No Hit
AAGGCTGAGGCGTGATGACGAGGCACNNNNNNNNN437750.49292513089400236No Hit
AAGGCTGAGGCGTGATGACGAGGNNNNNNNNNNNN425100.4786806925026622No Hit
GAGAGGACCGGAGTGGACGCATNNNNNNNNNNNNN379990.4278849125948874No Hit
AGCGACTTATATTCTGTAGCNNNNNNNNNNNNNNN362130.40777379246292417No Hit
AACGGAGGAGCACGAAGGTTGGCNNNNNNNNNNNN343450.3867393174312852No Hit
AGCGGATGACTTGTGGCTGGGGNNNNNNNNNNNNN325660.36670702027856267No Hit
AGTGATCCGGTGGTTCTGAANNNNNNNNNNNNNNN312360.3517306542228454No Hit
GAGAGAACTCGGGTGAAGGAANNNNNNNNNNNNNN308230.347080098447649No Hit
ACGGTCCAGACTCCTACGGGAGGCAGNNNNNNNNN287230.3234332046754638No Hit
AAGGTAGCGAAATTCCTTGTCGGGNNNNNNNNNNN284530.32039288976189717No Hit
ACTGGAACTGAGACACGGTCNNNNNNNNNNNNNNN270170.3042229185919649No Hit
ACGAGAGGACCGGAGTGGACNNNNNNNNNNNNNNN252550.2843820486745409No Hit
AGGCTGAGGCGTGATGACGANNNNNNNNNNNNNNN231140.2602734774525179No Hit
GAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTTG208160.23439701941038385No Hit
AAGGCGCTTGAGAGAACTCGGGNNNNNNNNNNNNN200510.22578279382194497No Hit
AGGATGTTGGCTTAGAAGCTNNNNNNNNNNNNNNN199860.2250508661575678No Hit
ACTGGTGTTCGGGTTGTCATGCNNNNNNNNNNNNN195990.22069308144812227No Hit
CATATCGACGGCGGTGTTTGGCACNNNNNNNNNNN194490.21900401760725188No Hit
ACGGTGCTGAAGCAACAAATGCNNNNNNNNNNNNN186850.2104010524444188No Hit
TATCGACGGCGGTGTTTGGCACNNNNNNNNNNNNN181650.20454563112940155No Hit
ATAACGGTCCTAAGGTAGCTNNNNNNNNNNNNNNN181320.20417403708441004No Hit
AAGTACCCCGAGGAAAAGAAATNNNNNNNNNNNNN181010.20382496389063018No Hit
TGGCCAGGCTGTCTCCACCCGAGNNNNNNNNNNNN178920.20147153493901745No Hit
AGATGTGAAATCCCCGGGCTNNNNNNNNNNNNNNN177040.19935457492512662No Hit
ATATCGACGGCGGTGTTTGGCACNNNNNNNNNNNN171410.19301495530905985No Hit
CGGATGACTTGTGGCTGGGGNNNNNNNNNNNNNNN167120.1881842327241706No Hit
AATGAACCGTGAGGCTTAACCNNNNNNNNNNNNNN166580.18757616974145727No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG163090.1836462812050322RNA PCR Primer, Index 4 (100% over 35bp)
TGCGTTCCGACGTTCAGGCTGCNNNNNNNNNNNNN148350.1670484138620794No Hit
TTGAGAGAACTCGGGTGAAGGAACNNNNNNNNNNN147670.16628270492088482No Hit
AGAGGGGGGTAGAATTCCAGGTGNNNNNNNNNNNN147540.16613631938800938No Hit
GTATCCTGTCTGAATATGGGGGGNNNNNNNNNNNN144010.16216138914916112No Hit
ATCCTGTCTGAATATGGGGGGACNNNNNNNNNNNN136540.15374985122162668No Hit
ATCTCCGGGGGTAGAGCACTGTTTNNNNNNNNNNN132010.14864887842219815No Hit
ATCTGCCGTGGGCGCTGGAGAANNNNNNNNNNNNN131280.1478268673529746No Hit
GGCTGAGGCGTGATGACGAGNNNNNNNNNNNNNNN130300.14672334564360595No Hit
AACGTCCGTCGTGAAGAGGGAAANNNNNNNNNNNN129880.14625040776816225No Hit
AAGGCTGAGGCGTGATGACGANNNNNNNNNNNNNN127940.14406588520063657No Hit
ATAGTGATCCGGTGGTTCTGAANNNNNNNNNNNNN126390.1423205192317372No Hit
ATATCGACGGCGGTGTTTGGCACCTNNNNNNNNNN125750.14159985199296585No Hit
ACGGTGCTGAAGCAACAAATGCCNNNNNNNNNNNN119010.134010325134655No Hit
AACTGGAGGACCGAACCGACNNNNNNNNNNNNNNN118990.13398780428344337No Hit
GGGGCTGATTCTGGATTCGACGNNNNNNNNNNNNN115780.1303732076639808No Hit
ACGCGAGCTGGGTTTAGAACNNNNNNNNNNNNNNN113920.12827876850130154No Hit
GAATTCATCTCCGGGGGTAGAGNNNNNNNNNNNNN111570.12563256848393797No Hit
ATTCTGTAGCAAGGTTAACCGAANNNNNNNNNNNN111130.12513710975728265No Hit
GGCTGAGGCGTGATGACGAGGNNNNNNNNNNNNNN110260.12415745272957783No Hit
ATGCGTTCCGACGTTCAGGCTGCNNNNNNNNNNNN108100.12172520079872451No Hit
ATCAGGAGGTTAGTGCAATGGNNNNNNNNNNNNNN106990.12047529355648043No Hit
GATGACTTGTGGCTGGGGGTNNNNNNNNNNNNNNN103940.11704086374671069No Hit
AAGGCTGAGGCGTGATGACGAGGCNNNNNNNNNNN103730.11680439480898884No Hit
GGGGCTGATTCTGGATTCGACGGGNNNNNNNNNNN101790.11461987224146315No Hit
AGGCCGGGTGTGTAAGCGCTNNNNNNNNNNNNNNN100820.11352761095770032No Hit
AATGATGGCCAGGCTGTCTCCACCCGAGACTCAGT99760.11233400584348527No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCAGNNNN99310.11182728669122415No Hit
ACTGTTTCGGCAAGGGGGTCNNNNNNNNNNNNNNN99070.11155703647668488No Hit
GGGGCTGATTCTGGATTCGACGGGANNNNNNNNNN96490.10865184667038785No Hit
AAGGCTGAGGCGTGATGACGAGGCANNNNNNNNNN95130.10712042878799873No Hit
CGAGACTCAGTGAAATTGAANNNNNNNNNNNNNNN92200.10382112408549862No Hit
TAACGGTCCTAAGGTAGCTGNNNNNNNNNNNNNNN92090.10369725940383477No Hit
GGGGCTGATTCTGGAATTCTNNNNNNNNNNNNNNN92040.10364095727580576No Hit
AGCTCACTGGTCGAGTCGGCCTGCGNNNNNNNNNN91380.10289776918582282No Hit
CACTAACTGGAGGACCGAACNNNNNNNNNNNNNNN89160.10039795470133467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)