FastQCFastQC Report
Mon 3 Jul 2023
SRR5266620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5266620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17442287
Total Bases610.4 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACTGG1986781.1390593446834123No Hit
GAGAGAACTCGGGTGAAGGAACTTGGAATTCTCGG1823891.0456713617887379No Hit
AAGGTAGCGAAATTCCTTGTCGGGTTGGAATTCTC1442880.8272309703423639No Hit
AGCGAAATTCCTTGTCGGGTTGGAATTCTCGGGTG1431870.820918724706227No Hit
GAGAGGACCGGAGTGGACGCTGGAATTCTCGGGTG1022310.5861100668736846No Hit
AGAGGGGGGTAGAATTCCAGGTGTTGGAATTCTCG973070.5578798239015331No Hit
AAGGCTGAGGCGTGATGACGAGGCACTTGGAATTC874430.5013276068671499No Hit
ACGAGAGGACCGGAGTGGACGCTGGAATTCTCGGG856530.4910651911644384No Hit
GAGAGGACCGGAGTGGACGCATCTGGAATTCTCGG802910.4603238096013441No Hit
AAGGCGCTTGAGAGAACTCGGGTTGGAATTCTCGG727660.41718153129804597No Hit
AACGGAGGAGCACGAAGGTTGGCTTGGAATTCTCG705630.4045513068326418No Hit
AGCTCACTGGTCGAGTCGGCCTGCGCTGGAATTCT637290.3653706649821781No Hit
ACTAACTGGAGGACCGAACCGACTTGGAATTCTCG605160.3469499154554675No Hit
TTGAGAGAACTCGGGTGAAGGAACTTGGAATTCTC583000.33424515947937333No Hit
GAGAGAACTCGGGTGAAGGAACTGGAATTCTCGGG580840.33300678976329195No Hit
ACTGGAACTGAGACACGGTCCTGGAATTCTCGGGT579430.33219840953196106No Hit
AACGTCCGTCGTGAAGAGGGAAACTGGAATTCTCG559970.32104161570096856No Hit
AGGCTGAGGCGTGATGACGAGGCACTTGGAATTCT554530.3179227586382451No Hit
AGTGATCCGGTGGTTCTGAATTGGAATTCTCGGGT534490.30643343960571223No Hit
ATTCTGTAGCAAGGTTAACCGAATTGGAATTCTCG523580.3001785259008753No Hit
AGCGACTTATATTCTGTAGCTGGAATTCTCGGGTG487500.2794931650878122No Hit
GAAGGTTGGCTAATCCTGGTCGGACTGGAATTCTC473530.2714838942851932No Hit
ATCCTGTCTGAATATGGGGGGACCTGGAATTCTCG448780.25729424128842737No Hit
ATAGTGATCCGGTGGTTCTGAATTGGAATTCTCGG438980.25167571202102107No Hit
AATGGGTCAGCGACTTATATTCTGTAGCTGGAATT432570.248000735224687No Hit
AACTGGAGGACCGAACCGACTTGGAATTCTCGGGT396150.22712044584520366No Hit
ACTCCTGACTGACCGATAGTGAACCTGGAATTCTC396020.2270459143345136No Hit
AACGGATAAAAGGTACTCCGGGGATTGGAATTCTC385480.22100312877548683No Hit
ACTGGTGTTCGGGTTGTCATGCCTGGAATTCTCGG365360.20946794419791392No Hit
ATGAAGAAGGCCTTCGGGTTGTAAAGTTGGAATTC355340.20372328468164755No Hit
ATCTCCGGGGGTAGAGCACTGTTTCTGGAATTCTC353790.20283463974649654No Hit
TGGCCAGGCTGTCTCCACCCGAGATGGAATTCTCG352080.20185426372126544No Hit
GAAAAAGAACCTGAAACCGTGTACGTACTGGAATT351340.20143000742964498No Hit
AGATTGAACGCTGGCGGCAGGCCTTGGAATTCTCG348570.1998419129326332No Hit
ATGGGCAGGTTGAAGGTTGGGTAACTGGAATTCTC347240.19907939824634233No Hit
AATGAACCGTGAGGCTTAACCTTGGAATTCTCGGG336110.19269835429264523No Hit
ACGGTCCAGACTCCTACGGGAGGCAGCTGGAATTC329370.18883418212302092No Hit
AGGTGGGAGGCTTTGAAGTGTGGACGCCTGGAATT310760.178164709708079No Hit
AGGATGTTGGCTTAGAAGCTGGAATTCTCGGGTGC282020.16168751265244058No Hit
TGCGTTCCGACGTTCAGGCTGCTTGGAATTCTCGG274540.1573990841911958No Hit
AGATGTGAAATCCCCGGGCTCTGGAATTCTCGGGT271690.15576512414914395No Hit
AGATTGAACGCTGGCGGCTGGAATTCTCGGGTGCC269010.15422862839030224No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCTGGAATT266900.1530189246398709No Hit
ATGCGTTCCGACGTTCAGGCTGCTTGGAATTCTCG263400.15101230704436866No Hit
AGCCATGGGCAGGTTGAAGGTTGGGTAACTGGAAT243030.13933379263854564No Hit
AGGTGCTGCATGGCTGTCGTCAGCTCTGGAATTCT241170.13826741871636444No Hit
ACGTAGACTGCCCGGGGCACGCCGATGGAATTCTC235770.13517149442616097No Hit
AAATGCGGAAGAGATAAGTGCTGAAAGCTGGAATT233270.13373819614365937No Hit
ATCAGGAGGTTAGTGCAATGGCTGGAATTCTCGGG228100.13077413529544607No Hit
ATCAGGAGGTTAGTGCAATGGCATTGGAATTCTCG217150.12449628881808905No Hit
AAGGCTGAGGCGTGATGACGAGGTGGAATTCTCGG215690.12365924262110811No Hit
AAGTACCCCGAGGAAAAGAAATCTGGAATTCTCGG211990.12153796116300575No Hit
ATATCGACGGCGGTGTTTGGCACCTCTGGAATTCT211610.12132009982406551No Hit
AACGGATAAAAGGTACTCCGGGGATAACTGGAATT209100.1198810683484339No Hit
ATGGCTCAGATTGAACGCTGGCGGCTGGAATTCTC209070.11986386876904388No Hit
AGTCCGGATTGGAGTCTGCAACTCTGGAATTCTCG198420.11375801808558707No Hit
GGAATCGCTAGTAATCGTGGATCTGGAATTCTCGG198210.11363762102985693No Hit
GATGGCCAGGCTGTCTCCACCCGAGACTCTGGAAT196910.1128923059229561No Hit
AAGGCTGAGGCGTGATGACGAGGCTGGAATTCTCG194560.11154500553740458No Hit
AGGATGTTGGCTTAGAAGCAGCCATCTGGAATTCT191670.10988811272283273No Hit
TGAGAGAACTCGGGTGAAGGAACTTGGAATTCTCG184900.1060067409738184No Hit
CTGACTGACCGATAGTGAACCAGTTGGAATTCTCG182290.10451037756688673No Hit
GTATCCTGTCTGAATATGGGGGGATGGAATTCTCG177520.10177564444387367No Hit
ATATTCTGTAGCAAGGTTAACCGAATTGGAATTCT176750.10133418857286318No Hit
CGGGGGTAGAGCACTGTTTCGGCTGGAATTCTCGG175690.1007264701010825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)