FastQCFastQC Report
Mon 10 Jul 2023
SRR5278491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5278491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112774341
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1204947610.684590034536313No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT72131336.396076391171286No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA62443625.537041444560514No Hit
TTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT34840503.0893995647467363No Hit
AAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA27512162.43957621530238No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT13630531.2086552560746067No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGTA8278710.7340951786186896No Hit
TTAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA8109160.719060730312758No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6523800.5784826532482242Illumina PCR Primer Index 6 (96% over 29bp)
TTTGGGCACTCTAAGGTGACTGCCGGTGCTGTAGGCACCATCAATATCTC5904470.52356501910306No Hit
CAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA5762560.5109814829243826No Hit
ATGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT5384990.47750134935392796No Hit
TTTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTA4932210.4373521455558761No Hit
CAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT4899340.43443747545374706No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTATG4837220.4289291302531309No Hit
TTGGGCACTCTAAGGTGACTGCCGGTGCTGTAGGCACCATCAATATCTCG4181400.3707758310021958No Hit
GAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT4154860.3684224587931753No Hit
TTAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGT3627840.3216901972408777No Hit
CTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT3391200.3007067006492195No Hit
TTGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT3240440.2873384114920255No Hit
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATATCTCGTAT3222710.285766245355404No Hit
GAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA3146180.27898012722592636No Hit
AAGAACCTTACCAGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC2867420.2542617384924466RNA PCR Primer, Index 6 (95% over 23bp)
TGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTATG2458990.21804516685227185No Hit
GCCGGTGTAGCTCAATTGGTAGAGCAACTGCTGTAGGCACCATCAATATC2302630.20418031083861535No Hit
TTTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCG1892300.16779526115785506No Hit
AGGTGCGGCTGGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC1847060.16378371033886158Illumina PCR Primer Index 6 (95% over 23bp)
AGGTGCGGCTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT1789330.15866463808465084RNA PCR Primer, Index 6 (95% over 22bp)
GTGCCTCTTGGGGTTGTAGGACACCTGTAGGCACCATCAATATCTCGTAT1785640.15833743599530323No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATATCTCGTATG1666380.1477623354057108No Hit
ATAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTA1576030.139750761212606No Hit
TTGCCTCTTGGGGTTGTAGGACACTGTAGGCACCATCAATATCTCGTATG1544750.13697708062865116No Hit
TAAGCGAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGC1538910.13645923233548313No Hit
AATGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATATCTCGTAT1382430.12258373560347384No Hit
CTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATATCTCGT1287760.11418909554966941No Hit
ACTACCGTGCCGGTTCAAGTCCGGCCCTCGGCACCACTGTAGGCACCATC1282550.11372711102785339No Hit
TAAGGTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTCTGTAGGCACCAT1208620.10717154179601901No Hit
TCCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATATCTCGTAT1143620.10140781935493641No Hit
TTTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATATCTCGT1133050.10047054941336345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)